PMID- 23558921
OWN - NLM
STAT- MEDLINE
DCOM- 20131113
LR  - 20211203
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 228
IP  - 10
DP  - 2013 Oct
TI  - KLF4 promoted odontoblastic differentiation of mouse dental papilla cells via 
      regulation of DMP1.
PG  - 2076-85
LID - 10.1002/jcp.24377 [doi]
AB  - Odontoblasts, which derive from dental papilla, are a type of terminally 
      differentiated matrix-secreting cells. Previous studies have identified various 
      transcription factors involved in the differentiation process of odontoblasts. We 
      have recently found that Kruppel-like factor 4 (Klf4) was expressed in the 
      polarizing and elongating odontoblasts, but the function of Klf4 in the 
      differentiation of odontoblasts is still unclear. We hypothesized Klf4 promoted 
      the differentiation of odontoblasts by up-regulating some odontoblast-related 
      genes. In this study, we found that the expression of Klf4 increased 
      significantly during the odontoblastic differentiation of primary mouse dental 
      papilla cells and the mouse dental papilla cell line-mDPC6T. Overexpression of 
      Klf4 significantly up-regulated odontoblast-related genes, such as Dmp1, Dspp, 
      and Alp, and promoted the accumulation of mineral nodules. Knock-down of Klf4 
      down-regulated expression of Dmp1, Dspp, and Alp, and inhibited mineral 
      deposition. We applied in silico analysis and identified one target gene of 
      Klf4-Dmp1. Based on further analysis of ChIP data, EMSA and dual luciferase 
      activity assays, we confirmed that Klf4 was able to specifically bind to the Dmp1 
      promoter and transactivate its expression. Furthermore, forced expression of Dmp1 
      in the Klf4 knock-down mDPC6T cell line significantly recovered its odontoblastic 
      differentiation ability. Our data confirmed our hypothesis that Klf4 promotes the 
      differentiation of odontoblasts via the up-regulation of Dmp1.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Lin, Heng
AU  - Lin H
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), 
      School and Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Liu, Huan
AU  - Liu H
FAU - Sun, Qin
AU  - Sun Q
FAU - Yuan, Guohua
AU  - Yuan G
FAU - Zhang, Lu
AU  - Zhang L
FAU - Chen, Zhi
AU  - Chen Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Dmp1 protein, mouse)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Klf4 protein, mouse)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Cell Line
MH  - Dental Papilla/cytology/*metabolism
MH  - Down-Regulation
MH  - Extracellular Matrix Proteins/genetics/*metabolism
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/genetics/*metabolism
MH  - Mice
MH  - Odontoblasts/cytology/*metabolism
MH  - Promoter Regions, Genetic
MH  - Up-Regulation
EDAT- 2013/04/06 06:00
MHDA- 2013/11/14 06:00
CRDT- 2013/04/06 06:00
PHST- 2013/01/08 00:00 [received]
PHST- 2013/03/25 00:00 [accepted]
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2013/11/14 06:00 [medline]
AID - 10.1002/jcp.24377 [doi]
PST - ppublish
SO  - J Cell Physiol. 2013 Oct;228(10):2076-85. doi: 10.1002/jcp.24377.