PMID- 23559197
OWN - NLM
STAT- MEDLINE
DCOM- 20130926
LR  - 20130405
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 990
DP  - 2013
TI  - Spreading and fluorescence in situ hybridization of male and female meiocyte 
      chromosomes from Arabidopsis thaliana for cytogenetical analysis.
PG  - 3-11
LID - 10.1007/978-1-62703-333-6_1 [doi]
AB  - Advances in molecular biology and in the genetics of Arabidopsis thaliana have 
      led to it becoming an important model for the analysis of meiosis in plants. 
      Cytogenetic investigations are pivotal to meiotic studies and a number of 
      technological improvements for Arabidopsis cytology have provided a range of 
      tools to investigate chromosome behavior during meiosis. This chapter contains a 
      detailed description of cytological techniques currently used in our laboratory 
      for the basic preparation of meiotic chromosomes for investigation of the female 
      and male meiotic pathway and fluorescence in situ hybridization (FISH) analysis 
      for the frequency and distribution of crossovers (chiasmata) at metaphase I.
FAU - Armstrong, Susan
AU  - Armstrong S
AD  - School of Biosciences, The University of Birmingham, Birmingham, UK.
LA  - eng
GR  - Biotechnology and Biological Sciences Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Arabidopsis/*cytology/*genetics
MH  - *Chromosomes, Plant
MH  - *Germ Cells, Plant
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Meiosis/*genetics
EDAT- 2013/04/06 06:00
MHDA- 2013/09/27 06:00
CRDT- 2013/04/06 06:00
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2013/09/27 06:00 [medline]
AID - 10.1007/978-1-62703-333-6_1 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;990:3-11. doi: 10.1007/978-1-62703-333-6_1.