PMID- 23559198
OWN - NLM
STAT- MEDLINE
DCOM- 20130926
LR  - 20130405
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 990
DP  - 2013
TI  - Analysis of plant meiotic chromosomes by chromosome painting.
PG  - 13-24
LID - 10.1007/978-1-62703-333-6_2 [doi]
AB  - Chromosome painting (CP) refers to visualization of large chromosome regions, 
      entire chromosome arms, or entire chromosomes via fluorescence in situ 
      hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial 
      artificial chromosomes (BAC) from the target species or from a closely related 
      species (comparative chromosome painting, CCP) are typically applied as painting 
      probes. Extended pachytene chromosomes provide the highest resolution of CP in 
      plants. CP enables identification and tracing of particular chromosome regions 
      and/or entire chromosomes throughout all meiotic stages as well as corresponding 
      chromosome territories in premeiotic interphase nuclei. Meiotic pairing and 
      structural chromosome rearrangements (typically inversions and translocations) 
      can be identified by CP. Here, we describe step-by-step protocols of CP and CCP 
      in plant species including chromosome preparation, BAC DNA labeling, and 
      multicolor FISH.
FAU - Lysak, Martin A
AU  - Lysak MA
AD  - Laboratory of Plant Cytogenomics, Central European Institute of Technology 
      (CEITEC), Masaryk University, Brno, Czech Republic.
FAU - Mandakova, Terezie
AU  - Mandakova T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Chromosome Painting/*methods
MH  - *Chromosomes, Plant
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Meiosis/*genetics
EDAT- 2013/04/06 06:00
MHDA- 2013/09/27 06:00
CRDT- 2013/04/06 06:00
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2013/09/27 06:00 [medline]
AID - 10.1007/978-1-62703-333-6_2 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;990:13-24. doi: 10.1007/978-1-62703-333-6_2.