PMID- 23559202
OWN - NLM
STAT- MEDLINE
DCOM- 20130926
LR  - 20131121
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 990
DP  - 2013
TI  - Three-dimensional acrylamide fluorescence in situ hybridization for plant cells.
PG  - 53-66
LID - 10.1007/978-1-62703-333-6_6 [doi]
AB  - Plant meiosis involves complex and dynamic processes that occur within the space 
      inside the nucleus. Direct inspection of meiotic chromosomes by fluorescence 
      microscopy has been used to investigate many of these processes. In particular, 
      optical sectioning microscopy of fluorescence in situ hybridization 
      (FISH)-stained nuclei provides three-dimensional spatial information about the 
      organization and distribution of specific sequences and chromosomal loci within 
      the nucleus. Here we provide a fully detailed three-dimensional (3D) acrylamide 
      FISH method for the analysis of plant meiotic nuclei. Several examples illustrate 
      the versatility of this technique for the investigation of meiotic telomere 
      dynamics in maize, Arabidopsis, and oat. Additional examples of 3D FISH include 
      chromosome painting in a maize chromosome-addition line of oat and telomere FISH 
      with maize nuclei from plants expressing a fluorescently tagged fusion protein, 
      histone H2B-mCherry.
FAU - Howe, Elizabeth S
AU  - Howe ES
AD  - Department of Biological Science, The Florida State University, Tallahassee, FL, 
      USA.
FAU - Murphy, Shaun P
AU  - Murphy SP
FAU - Bass, Hank W
AU  - Bass HW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 20R035KLCI (Acrylamide)
SB  - IM
MH  - Acrylamide/chemistry
MH  - Chromosomes, Plant
MH  - Germ Cells, Plant
MH  - Imaging, Three-Dimensional
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Meiosis/genetics
MH  - *Plant Cells
EDAT- 2013/04/06 06:00
MHDA- 2013/09/27 06:00
CRDT- 2013/04/06 06:00
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2013/09/27 06:00 [medline]
AID - 10.1007/978-1-62703-333-6_6 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;990:53-66. doi: 10.1007/978-1-62703-333-6_6.