PMID- 23559854
OWN - NLM
STAT- MEDLINE
DCOM- 20130924
LR  - 20220409
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 19
DP  - 2013
TI  - Increased importin 13 activity is associated with the pathogenesis of pterygium.
PG  - 604-13
AB  - PURPOSE: We previously reported that importin 13 (IPO13), a member of the 
      importin-beta family of nuclear import proteins, regulates nuclear import of the 
      glucocorticoid receptor in airway epithelial cells, IPO13 serves as a potential 
      marker for corneal epithelial progenitor cells, and IPO13 is associated with 
      corneal cell proliferation. Here we investigated the role of IPO13 in the 
      pathogenesis of pterygium and the underlying mechanism including interaction with 
      other cell proliferation-related factors: keratin 17 (K17), a lesional protein 
      and a member of the type I keratins, and c-Jun, a protein of the activator 
      protein-1 complex. METHODS: Tissue samples were collected from primary pterygia, 
      recurrent pterygia, and normal conjunctiva to perform the following experiments: 
      immunohistochemical measurement of IPO13 and K17. Pterygium epithelial cells 
      (PECs) were cultured in keratinocyte serum-free defined medium to examine the 
      expression of IPO13 and K17. Lentivirus-mediated silencing and overexpression 
      IPO13 testing was conducted, and K17 alternation was evaluated with western blot 
      and immunostaining. In addition, the translocation of c-Jun (a K17 regulator) was 
      further examined after IPO13 was silenced. RESULTS: IPO13 activity was 
      significantly increased in the basal layer of the epithelium of the pterygium. In 
      cultured PECs, overexpression or knockdown of the IPO13 gene increased or 
      decreased PEC proliferation, respectively. IPO13 was colocalized with K17 in the 
      epithelium of the pterygium, and overexpression or knockdown of the IPO13 gene 
      induced upregulation or downregulation of K17 expression in PECs, respectively. 
      In addition, silencing of the IPO13 gene blocked nuclear translocation of c-Jun. 
      CONCLUSIONS: We provided novel evidence that IPO13 may contribute to the 
      pathogenesis of pterygium via modulation of K17 and c-Jun.
FAU - Xu, Ke
AU  - Xu K
AD  - Eye Institute of Xiamen University, Fujian Provincial Key Laboratory of 
      Ophthalmology and Visual Science, Xiamen, China.
FAU - Tao, Tao
AU  - Tao T
FAU - Jie, Jing
AU  - Jie J
FAU - Lu, Xiaodong
AU  - Lu X
FAU - Li, Xuezhi
AU  - Li X
FAU - Mehmood, Muhammad Aamer
AU  - Mehmood MA
FAU - He, Hui
AU  - He H
FAU - Liu, Zhen
AU  - Liu Z
FAU - Xiao, Xinye
AU  - Xiao X
FAU - Yang, Jie
AU  - Yang J
FAU - Ma, Jian-xing
AU  - Ma JX
FAU - Li, Wei
AU  - Li W
FAU - Zhou, Yueping
AU  - Zhou Y
FAU - Liu, Zuguo
AU  - Liu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130320
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (IPO13 protein, human)
RN  - 0 (Karyopherins)
RN  - 0 (Keratin-17)
RN  - 0 (Transcription Factor AP-1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cell Nucleus/metabolism
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Epithelial Cells/metabolism/pathology
MH  - Epithelium/metabolism/pathology
MH  - Female
MH  - Gene Expression Regulation
MH  - Gene Silencing
MH  - Humans
MH  - Karyopherins/genetics/*metabolism
MH  - Keratin-17/genetics/metabolism
MH  - Male
MH  - Middle Aged
MH  - Protein Transport
MH  - Pterygium/*etiology/genetics/*metabolism/pathology
MH  - Transcription Factor AP-1/metabolism
PMC - PMC3611950
EDAT- 2013/04/06 06:00
MHDA- 2013/09/26 06:00
PMCR- 2013/01/01
CRDT- 2013/04/06 06:00
PHST- 2012/10/08 00:00 [received]
PHST- 2013/03/18 00:00 [accepted]
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2013/09/26 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 63 [pii]
PST - ppublish
SO  - Mol Vis. 2013;19:604-13. Epub 2013 Mar 20.