PMID- 23561173 OWN - NLM STAT- MEDLINE DCOM- 20131018 LR - 20130408 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 139 IP - 1-4 DP - 2013 Aug 15 TI - Chemical properties of omega-3 fortified gels made of protein isolate recovered with isoelectric solubilisation/precipitation from whole fish. PG - 777-85 LID - S0308-8146(13)00112-X [pii] LID - 10.1016/j.foodchem.2013.01.077 [doi] AB - Protein isolate was recovered from whole gutted fish using isoelectric solubilisation/precipitation (ISP). The objective was to determine chemical properties of heat-set gels made of the ISP protein isolate fortified with omega-3 polyunsaturated fatty acids (PUFAs)-rich oils (flaxseed, fish, algae, krill, and blend). The extent of the PUFAs increase, omega-6/omega-3 FAs and unsaturated/saturated FAs ratios, and the indices of thrombogenicity and atherogenicity depended on specific omega-3 PUFAs-rich oil used to fortify protein isolate gels. Lipid oxidation in omega-3 PUFAs fortified gels was minimal, although greater (P<0.05) than control gels (without omega-3 PUFAs fortification). However, all gels were in the slightly rancid, but acceptable range. The commonly used thiobarbituric-acid-reactive-substances (TBARS) assay to determine lipid oxidation in seafood may be inaccurate for samples containing krill oil due to its red pigment, astaxanthin. Protein degradation (total-volatile-basic-nitrogen) was greater (P<0.05) in omega-3 PUFAs fortified gels than control gels. However, all gels were considerably below the acceptability threshold for protein degradation. The shear stress of omega-3 PUFAs fortified gels was generally greater than the control gels and the shear strain was generally unchanged. This study demonstrates that omega-3 PUFAs fortification of protein isolates recovered with ISP from fish processing by-products or whole fish has potential application in the development of functional foods. CI - Copyright (c) 2013 Elsevier Ltd. All rights reserved. FAU - Tahergorabi, Reza AU - Tahergorabi R AD - Oregon State University, Seafood Research and Education Center, 2001 Marine Dr., Astoria, OR 97103, USA. FAU - Beamer, Sarah K AU - Beamer SK FAU - Matak, Kristen E AU - Matak KE FAU - Jaczynski, Jacek AU - Jaczynski J LA - eng PT - Journal Article DEP - 20130209 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 RN - 0 (Fatty Acids, Omega-3) RN - 0 (Fish Proteins) RN - 0 (Gels) RN - 0 (Lipids) RN - 0 (Protein Hydrolysates) SB - IM MH - Animals MH - Chemical Precipitation MH - Fatty Acids, Omega-3/*analysis MH - Fish Products/*analysis MH - Fish Proteins/*chemistry/isolation & purification MH - Fishes MH - Food Handling MH - Functional Food/*analysis MH - Gels/chemistry MH - Lipids/chemistry MH - Oxidation-Reduction MH - Protein Hydrolysates/chemistry/isolation & purification MH - Solubility EDAT- 2013/04/09 06:00 MHDA- 2013/10/19 06:00 CRDT- 2013/04/09 06:00 PHST- 2012/08/08 00:00 [received] PHST- 2013/01/02 00:00 [revised] PHST- 2013/01/29 00:00 [accepted] PHST- 2013/04/09 06:00 [entrez] PHST- 2013/04/09 06:00 [pubmed] PHST- 2013/10/19 06:00 [medline] AID - S0308-8146(13)00112-X [pii] AID - 10.1016/j.foodchem.2013.01.077 [doi] PST - ppublish SO - Food Chem. 2013 Aug 15;139(1-4):777-85. doi: 10.1016/j.foodchem.2013.01.077. Epub 2013 Feb 9.