PMID- 23562757
OWN - NLM
STAT- MEDLINE
DCOM- 20131203
LR  - 20130507
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 16
IP  - 1
DP  - 2013 May
TI  - Alkaloids from Galipea longiflora Krause modify the maturation of human dendritic 
      cells and their ability to stimulate allogeneic CD4+ T cells.
PG  - 79-84
LID - S1567-5769(13)00116-1 [pii]
LID - 10.1016/j.intimp.2013.03.022 [doi]
AB  - Alkaloids obtained from the plant Evanta have been shown to have dual effects in 
      Leishmania infection; a direct leishmanicidal effect on the parasite and more 
      importantly, the alkaloids affect both polyclonal and Leishmania-specific 
      stimulation of T-cells. Dendritic cells (DCs) play a pivotal role in stimulation 
      and polarization of naive T cells towards a Th1, Th2, Th17 or regulatory 
      phenotype. In leishmaniasis, the interactions between the parasites and DCs are 
      complex and involve contradictory functions that can stimulate or suppress T cell 
      responses, leading to the control of infection or progression of disease. In this 
      study the effect of an alkaloid extract of Evanta (AEE) or the purified alkaloid 
      2-phenilquinoline (2Ph) on the activation of human DCs and their ability to 
      stimulate allogeneic CD4(+) T cells was analyzed. The expression of surface 
      activation molecules was not affected on DCs stimulated in the presence of AEE or 
      2Ph nor did AEE-DCs or 2Ph-CDs affect the expression of activation surface 
      molecules on allogeneic CD4(+) T cells. In contrast, as compared with control, 
      the secretion of IL-12p40, IL-23 and IL-6 was lower from AEE-DCs and 2Ph-CDs and 
      allogeneic CD4(+) T cells co-cultured with these DCs secreted lower levels of 
      IFN-gamma and IL-10 but the same levels of IL-17. These results demonstrate that AEE 
      and 2Ph affect the stimulation of DCs and their ability to stimulate allogeneic 
      CD4(+) T cells by reducing the production of IFN-gamma, IL-12 p40, IL-6 and IL-23. 
      This suggests that AEE and 2Ph may take part in regulation of inflammation.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Calla-Magarinos, Jacqueline
AU  - Calla-Magarinos J
AD  - Department of Immunology and Center for Rheumatology Research, Landspitali, The 
      National University Hospital of Iceland, Reykjavik, Iceland. 
      jaqueline.calla@imun.su.se
FAU - Fernandez, Carmen
AU  - Fernandez C
FAU - Troye-Blomberg, Marita
AU  - Troye-Blomberg M
FAU - Freysdottir, Jona
AU  - Freysdottir J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130402
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Alkaloids)
RN  - 0 (Cytokines)
RN  - 0 (Immunologic Factors)
SB  - IM
MH  - Alkaloids/*pharmacology
MH  - CD4-Positive T-Lymphocytes/*drug effects/immunology
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytokines/immunology
MH  - Dendritic Cells/cytology/*drug effects/immunology
MH  - Humans
MH  - Immunologic Factors/*pharmacology
MH  - *Rutaceae
EDAT- 2013/04/09 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/04/09 06:00
PHST- 2012/09/26 00:00 [received]
PHST- 2013/03/14 00:00 [revised]
PHST- 2013/03/15 00:00 [accepted]
PHST- 2013/04/09 06:00 [entrez]
PHST- 2013/04/09 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - S1567-5769(13)00116-1 [pii]
AID - 10.1016/j.intimp.2013.03.022 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2013 May;16(1):79-84. doi: 10.1016/j.intimp.2013.03.022. 
      Epub 2013 Apr 2.