PMID- 23563626
OWN - NLM
STAT- MEDLINE
DCOM- 20131112
LR  - 20171116
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 31
IP  - 6
DP  - 2013 Jun
TI  - Hydrogen-rich medium suppresses the generation of reactive oxygen species, 
      elevates the Bcl-2/Bax ratio and inhibits advanced glycation end product-induced 
      apoptosis.
PG  - 1381-7
LID - 10.3892/ijmm.2013.1334 [doi]
AB  - The purpose of the present study was to determine whether using hydrogen-rich 
      medium (HRM) to increase hydrogen levels in endothelial cells (ECs) protects ECs 
      from apoptosis induced by advanced glycation end products (AGEs). The thoracic 
      aorta was removed from 2-3-year-old Sprague-Dawley rats, and ECs were isolated 
      and cultured. After culturing ECs in the presence of AGEs and/or with HRM for 
      24 h, Annexin V/7-AAD and TUNEL staining were carried out to detect apoptosis. 
      Intracellular ROS were detected by fluorescent probe and quantified by flow 
      cytometry. The expression of antioxidative enzymes (superoxide dismutase, 
      glutathione peroxidase) was determined by real-time PCR analysis and enzymatic 
      assay. The relative expression levels of Bcl-2 and Bax were analyzed by western 
      blotting. The addition of AGEs increased the apoptosis of ECs in a 
      concentration-dependent manner and HRM reduced the AGE (400 microg/ml)-induced 
      apoptosis from 21.61+/-2.52 to 11.32+/-1.75%. HRM also significantly attenuated the 
      AGE-induced intracellular ROS induction and decrease in the expression of 
      antioxidative enzymes. In conclusion, hydrogen exhibits significant protective 
      effects against AGE-induced EC injury possibly through reducing ROS generation, 
      intracellular antioxidant enzyme system protection and elevation of the Bcl-2/Bax 
      ratio.
FAU - Jiang, Hong
AU  - Jiang H
AD  - Third Military Medical University, Chongqing, People's Republic of China.
FAU - Yu, Pan
AU  - Yu P
FAU - Qian, De-Hui
AU  - Qian DH
FAU - Qin, Zhe-Xue
AU  - Qin ZX
FAU - Sun, Xue-Jun
AU  - Sun XJ
FAU - Yu, Jie
AU  - Yu J
FAU - Huang, Lan
AU  - Huang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130405
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cells, Cultured
MH  - Endothelial Cells/*drug effects/*metabolism
MH  - Glycation End Products, Advanced/pharmacology
MH  - Hydrogen/*pharmacology
MH  - Oxidative Stress/drug effects
MH  - Proto-Oncogene Proteins c-bcl-2/*metabolism
MH  - Rats
MH  - Reactive Oxygen Species/*metabolism
MH  - Superoxide Dismutase/metabolism
MH  - bcl-2-Associated X Protein/*metabolism
EDAT- 2013/04/09 06:00
MHDA- 2013/11/13 06:00
CRDT- 2013/04/09 06:00
PHST- 2012/12/20 00:00 [received]
PHST- 2013/02/20 00:00 [accepted]
PHST- 2013/04/09 06:00 [entrez]
PHST- 2013/04/09 06:00 [pubmed]
PHST- 2013/11/13 06:00 [medline]
AID - 10.3892/ijmm.2013.1334 [doi]
PST - ppublish
SO  - Int J Mol Med. 2013 Jun;31(6):1381-7. doi: 10.3892/ijmm.2013.1334. Epub 2013 Apr 
      5.