PMID- 23564007
OWN - NLM
STAT- MEDLINE
DCOM- 20140225
LR  - 20211021
IS  - 1436-2236 (Electronic)
IS  - 1436-2228 (Linking)
VI  - 15
IP  - 5
DP  - 2013 Oct
TI  - Phylogenetically diverse denitrifying and ammonia-oxidizing bacteria in corals 
      Alcyonium gracillimum and Tubastraea coccinea.
PG  - 540-51
LID - 10.1007/s10126-013-9503-6 [doi]
AB  - To date, the association of coral-bacteria and the ecological roles of bacterial 
      symbionts in corals remain largely unknown. In particular, little is known about 
      the community components of bacterial symbionts of corals involved in the process 
      of denitrification and ammonia oxidation. In this study, the nitrite reductase 
      (nirS and nirK) and ammonia monooxygenase subunit A (amoA) genes were used as 
      functional markers. Diverse bacteria with the potential to be active as 
      denitrifiers and ammonia-oxidizing bacteria (AOB) were found in two East China 
      Sea corals: stony coral Alcyonium gracillimum and soft coral Tubastraea coccinea. 
      The 16S rRNA gene library analysis demonstrated different communities of 
      bacterial symbionts in these two corals of the same location. Nitrite reductase 
      nirK gene was found only in T. coccinea, while both nirK and nirS genes were 
      detected in A. gracillimum, which might be the result of the presence of 
      different bacterial symbionts in these two corals. AOB rather than 
      ammonia-oxidizing archaea were detected in both corals, suggesting that AOB might 
      play an important role in the ammonia oxidation process of the corals. This study 
      indicates that the coral bacterial symbionts with the potential for nitrite 
      reduction and ammonia oxidation might have multiple ecological roles in the coral 
      holobiont, which promotes our understanding of bacteria-mediated nitrogen cycling 
      in corals. To our knowledge, this study is the first assessment of the community 
      structure and phylogenetic diversity of denitrifying bacteria and AOB in corals 
      based on nirK, nirS, and amoA gene library analysis.
FAU - Yang, Shan
AU  - Yang S
AD  - Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism, 
      Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China. 
      Yangshan1987@163.com
FAU - Sun, Wei
AU  - Sun W
FAU - Zhang, Fengli
AU  - Zhang F
FAU - Li, Zhiyong
AU  - Li Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130406
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
RN  - 0 (DNA Primers)
RN  - 0 (Genetic Markers)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.- (Nitrite Reductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Ammonia/metabolism
MH  - Animals
MH  - Anthozoa/*microbiology
MH  - Bacteria/*genetics/*metabolism
MH  - Base Sequence
MH  - China
MH  - Cluster Analysis
MH  - Computational Biology
MH  - DNA Primers/genetics
MH  - Denitrification/genetics/physiology
MH  - Gene Library
MH  - Genetic Markers/genetics
MH  - Microbiota/*genetics
MH  - Molecular Sequence Data
MH  - Nitrite Reductases/genetics
MH  - Oceans and Seas
MH  - Oxidation-Reduction
MH  - Oxidoreductases/genetics
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Species Specificity
MH  - *Symbiosis
EDAT- 2013/04/09 06:00
MHDA- 2014/02/26 06:00
CRDT- 2013/04/09 06:00
PHST- 2012/03/20 00:00 [received]
PHST- 2013/03/12 00:00 [accepted]
PHST- 2013/04/09 06:00 [entrez]
PHST- 2013/04/09 06:00 [pubmed]
PHST- 2014/02/26 06:00 [medline]
AID - 10.1007/s10126-013-9503-6 [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2013 Oct;15(5):540-51. doi: 10.1007/s10126-013-9503-6. Epub 
      2013 Apr 6.