PMID- 23564155
OWN - NLM
STAT- MEDLINE
DCOM- 20130813
LR  - 20130408
IS  - 0065-1419 (Print)
IS  - 0065-1419 (Linking)
VI  - 118
DP  - 2013
TI  - Hypnotic effect of volatile anesthetics is mediated by PKC-gamma dynamics.
PG  - 307-10
LID - 10.1007/978-3-7091-1434-6_60 [doi]
AB  - BACKGROUND: Although protein kinase C-gamma (PKC-gamma) is a target for the effects of 
      volatile anesthetics, the molecular mechanisms of the kinase function remain 
      unclear. We examined the effects of different types of anesthetics on PKC-gamma 
      knockout mice, and investigated the dynamics of the kinase in mouse brain. 
      METHODS: We measured the required number of times for loss of righting reflex 
      (rtfLORR) after administration of isoflurane, sevoflurane, and propofol on PKC-gamma 
      knockout mice and compared with those of wild-type mice. We also used 
      immunoblotting to investigate the intracellular distribution of PKC-gamma and 
      phosphorylated PKC-gamma (p-PKC-gamma) in brain of wild-type mice anesthetized by these 
      anesthetics. RESULTS: Isoflurane and sevoflurane significantly prolonged the 
      rtfLORRs in PKC-gamma knockout mice compared with those in wild-type mice, while no 
      significant difference was observed between knockout and wild-type mice treated 
      with propofol. Examination of the cellular fractions showed that PKC-gamma was 
      significantly decreased, whereas p-PKC-gamma was significantly increased in the 
      synaptic membrane fraction (P2). There was no significant change in the 
      supernatant fraction (S). In propofol-treated mice, PKC-gamma and p-PKC-gamma showed no 
      significant changes in P2 or S. CONCLUSION: Our results provide new evidence to 
      support the possibility of the involvement of PKC-gamma in the actions of volatile 
      anesthetics.
FAU - Takeda, Akiko
AU  - Takeda A
AD  - Department of Anesthesiology, Tokyo Medical University Hospital, Shinjuku-ku, 
      Tokyo, Japan. a-takeda@tokyo-med.ac.jp
FAU - Okita, Ayano
AU  - Okita A
FAU - Kaneko, Kouki
AU  - Kaneko K
FAU - Nagura, Takeo
AU  - Nagura T
FAU - Iwase, Naoto
AU  - Iwase N
FAU - Sekine, Shusuke
AU  - Sekine S
FAU - Kakinuma, Takayasu
AU  - Kakinuma T
FAU - Noguchi, Masashi
AU  - Noguchi M
FAU - Hatakeyama, Kiyoshi
AU  - Hatakeyama K
LA  - eng
PT  - Journal Article
PL  - Austria
TA  - Acta Neurochir Suppl
JT  - Acta neurochirurgica. Supplement
JID - 100962752
RN  - 0 (Anesthetics, Inhalation)
RN  - EC 2.7.1.- (protein kinase C gamma)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Anesthetics, Inhalation/*administration & dosage
MH  - Animals
MH  - Brain/*drug effects/*enzymology
MH  - Gene Expression Regulation, Enzymologic/drug effects/genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Phosphorylation/drug effects/genetics
MH  - Protein Kinase C/deficiency
MH  - Reflex, Righting/*drug effects/genetics
MH  - Time Factors
EDAT- 2013/07/24 06:00
MHDA- 2013/08/14 06:00
CRDT- 2013/04/09 06:00
PHST- 2013/04/09 06:00 [entrez]
PHST- 2013/07/24 06:00 [pubmed]
PHST- 2013/08/14 06:00 [medline]
AID - 10.1007/978-3-7091-1434-6_60 [doi]
PST - ppublish
SO  - Acta Neurochir Suppl. 2013;118:307-10. doi: 10.1007/978-3-7091-1434-6_60.