PMID- 23564227
OWN - NLM
STAT- MEDLINE
DCOM- 20131126
LR  - 20211021
IS  - 1559-0755 (Electronic)
IS  - 0257-277X (Linking)
VI  - 56
IP  - 1
DP  - 2013 May
TI  - House dust mite allergen Der f 2 induces interleukin-13 expression by activating 
      the PI3K/Akt pathway.
PG  - 181-8
LID - 10.1007/s12026-013-8392-0 [doi]
AB  - House dust mites (HDMs) are a common cause of allergic asthma. The group 2 
      allergen from Dermatophagoides farinae, Der f 2, is one of the major HDM 
      allergens. Elevated Der f 2 immunoglobulin E (IgE) levels are observed in most of 
      the allergic patients. Interleukin-13 (IL-13), a gene associated with asthma 
      pathology, was induced by Der f 2 in BEAS-2B human airway epithelial cells; 
      however, the signaling pathways associated with Der f 2 are not fully understood. 
      In this study, we identified a role of the phosphatidylinositol-3-kinase 
      (PI3K)/Akt pathway, a well-known potential target for anti-asthma drugs, in the 
      IL-13 induction by Der f 2. First, Der f 2 activated the PI3K/Akt pathway, which 
      subsequently activated the nuclear factor-kappa B (NF-kappaB) pathway and induced 
      IL-13 expression in BEAS-2B cells. Treatment with the PI3K inhibitor LY294002 
      abolished Der f 2-induced activation of Akt and NF-kappaB and the expression of 
      IL-13. Furthermore, Der f 2-induced activation of the PI3K/Akt and NF-kappaB 
      pathways, expression of IL-13, and the blockade of these effects with a PI3K 
      inhibitor were confirmed in the lungs of mice that were intranasally exposed to 
      Der f 2. Taken together, these results indicate that the PI3K/Akt pathway 
      regulates Der f 2-induced IL-13 expression via activation of the NF-kappaB pathway.
FAU - Ro, Eun Ji
AU  - Ro EJ
AD  - Translational Research Center for Protein Function Control, Department of 
      Biotechnology, College of Life Science and Biotechnology, Yonsei University, 
      Seoul, South Korea.
FAU - Cha, Pu-Hyeon
AU  - Cha PH
FAU - Kim, Hyun-Yi
AU  - Kim HY
FAU - Cho, Yong-Hee
AU  - Cho YH
FAU - Park, Jung-Won
AU  - Park JW
FAU - Han, Joong-Soo
AU  - Han JS
FAU - Choi, Kang-Yell
AU  - Choi KY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunol Res
JT  - Immunologic research
JID - 8611087
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Dermatophagoides farinae antigen f 2)
RN  - 0 (Interleukin-13)
RN  - 0 (NF-kappa B)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
SB  - IM
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Arthropod Proteins/*immunology
MH  - Asthma/*immunology
MH  - Cell Line
MH  - Gene Expression Regulation/immunology
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Interleukin-13/genetics/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - NF-kappa B/metabolism
MH  - Oncogene Protein v-akt/metabolism
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Pyroglyphidae/immunology
MH  - Respiratory Mucosa/*immunology
MH  - Signal Transduction/immunology
MH  - Transcriptional Activation/immunology
EDAT- 2013/04/09 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/04/09 06:00
PHST- 2013/04/09 06:00 [entrez]
PHST- 2013/04/09 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 10.1007/s12026-013-8392-0 [doi]
PST - ppublish
SO  - Immunol Res. 2013 May;56(1):181-8. doi: 10.1007/s12026-013-8392-0.