PMID- 23564567
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20181203
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 102
IP  - 3
DP  - 2014 Mar
TI  - Evaluation of in vitro and in vivo osteogenic differentiation of 
      nano-hydroxyapatite/chitosan/poly(lactide-co-glycolide) scaffolds with human 
      umbilical cord mesenchymal stem cells.
PG  - 760-8
LID - 10.1002/jbm.a.34747 [doi]
AB  - We aimed to evaluate the feasibility of the application of the 
      nano-hydroxyapatite/chitosan/poly(lactide-co-glycolide) (nHA/CS/PLGA) scaffold 
      seeded with human umbilical cord mesenchymal stem cells (hUCMSCs) in bone tissue 
      engineering. We prepared the nHA/CS/PLGA, nHA/PLGA, CS/PLGA, and PLGA scaffolds, 
      and tested their mechanical strength. We analyzed the surface antigen markers of 
      hUCMSCs to determine their capability to differentiate into osteoblasts, 
      chondrocytes, and adipocytes. The growth of hUCMSCs on the four types of scaffold 
      was assayed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium 
      bromide assay (MTT assay) and observed using scanning electron microscopy (SEM). 
      Quantitative analysis of alkaline phosphatase (ALP) activity and osteocalcin 
      (OCN) content, as well as the semi-quantitative reverse transcription-polymerase 
      chain reaction (RT-PCR) was performed. After 21 days, the subcutaneous 
      implantations of the scaffolds samples seeded with hUCMSCs into nude mice were 
      analyzed using immunohistochemical staining. The results showed that the 
      mechanical strength of the nHA/CS/PLGA scaffold was enhanced. Furthermore, the 
      nHA/CS/PLGA scaffolds were the most suitable for the adhesion, proliferation, and 
      osteogenic differentiation of hUCMSCs in vitro and nude mouse subcutaneous 
      implantation. The enhanced osteogenic inductivity of the nHA/CS/PLGA scaffolds 
      for hUCMSCs might result from the addition of nHA and CS.
CI  - Copyright (c) 2013 Society of Plastics Engineers.
FAU - Wang, Fei
AU  - Wang F
AD  - The Department of Orthodontics, the Affiliated Stomatological Hospital of Xi'an 
      Jiaotong University, Xian, 710004, China.
FAU - Zhang, Yin-Cheng
AU  - Zhang YC
FAU - Zhou, Hong
AU  - Zhou H
FAU - Guo, Yu-Cheng
AU  - Guo YC
FAU - Su, Xiao-Xia
AU  - Su XX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130601
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 34346-01-5 (Polyglactin 910)
RN  - 9012-76-4 (Chitosan)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chitosan/*chemistry
MH  - Durapatite/*chemistry
MH  - Humans
MH  - Infant
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Mice, Nude
MH  - Nanostructures/*chemistry/ultrastructure
MH  - Osteogenesis
MH  - Polyglactin 910/*chemistry
MH  - Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - bone marrow mesenchymal stem cells
OT  - bone tissue engineering
OT  - chitosan
OT  - nano-hydroxyapatite
OT  - poly(lactide-co-glycolide)
OT  - umbilical cord mesenchymal stem cells
EDAT- 2013/04/09 06:00
MHDA- 2014/09/16 06:00
CRDT- 2013/04/09 06:00
PHST- 2013/01/05 00:00 [received]
PHST- 2013/03/17 00:00 [revised]
PHST- 2013/04/02 00:00 [accepted]
PHST- 2013/04/09 06:00 [entrez]
PHST- 2013/04/09 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
AID - 10.1002/jbm.a.34747 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2014 Mar;102(3):760-8. doi: 10.1002/jbm.a.34747. Epub 2013 
      Jun 1.