PMID- 23564794
OWN - NLM
STAT- MEDLINE
DCOM- 20130611
LR  - 20151119
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 33
IP  - 4
DP  - 2013 Apr
TI  - Normal mammary fibroblasts induce reversion of the malignant phenotype in human 
      primary breast cancer.
PG  - 1525-36
AB  - BACKGROUND/AIM: The tumor microenvironment plays a major role in tumor growth and 
      progression. Its manipulation can lead to a reversion of the malignant phenotype. 
      Here we explored the ability of normal mammary fibroblasts (HMFs) to induce 
      reversion of the malignant phenotype of primary breast carcinoma cells (PBCs) in 
      a three-dimensional (3D) context. MATERIALS AND METHODS: PBCs were isolated from 
      13 primary breast carcinomas and cultured in 3D collagen-I gels as mono- or 
      co-culture with HMFs. RESULTS: In five co-cultures, PBCs exhibited reversion of 
      their malignant phenotype, whereas PBCs in matched monocultures exhibited 
      disorganized growth. Reversion, defined as the restoration of the complete 
      baso-apical polarity axis, was confirmed with established polarity markers. 
      Secretion of the tissue-specific glycoprotein MAM-6 into the acinar lumens and 
      deposition of basement membrane indicated functional differentiation. Gene 
      expression analysis revealed a set of differentially regulated genes which 
      possibly affect the reversion process. These included MAL, ELF5, MAP6, ZMYND11 
      and SQLE. CONCLUSION: These findings highlight the significant role of 
      fibroblasts in regulating the carcinoma phenotype.
FAU - Romer, Anna-Maria
AU  - Romer AM
AD  - Department of Gynecology and Obstetrics, University Medical Center 
      Schleswig-Holstein, Kiel, Germany.
FAU - Luhr, Inke
AU  - Luhr I
FAU - Klein, Andreas
AU  - Klein A
FAU - Friedl, Andreas
AU  - Friedl A
FAU - Sebens, Susanne
AU  - Sebens S
FAU - Rosel, Frank
AU  - Rosel F
FAU - Arnold, Norbert
AU  - Arnold N
FAU - Strauss, Alexander
AU  - Strauss A
FAU - Jonat, Walter
AU  - Jonat W
FAU - Bauer, Maret
AU  - Bauer M
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - Breast/*cytology/metabolism
MH  - Breast Neoplasms/metabolism/*pathology
MH  - Cell Communication
MH  - Cell Culture Techniques
MH  - *Cell Transformation, Neoplastic
MH  - Coculture Techniques
MH  - Female
MH  - Fibroblasts/*cytology/metabolism
MH  - Gene Expression Profiling
MH  - Humans
MH  - Neoplasm Invasiveness
MH  - Oligonucleotide Array Sequence Analysis
MH  - Phenotype
MH  - Tumor Cells, Cultured
MH  - *Tumor Microenvironment
EDAT- 2013/04/09 06:00
MHDA- 2013/06/12 06:00
CRDT- 2013/04/09 06:00
PHST- 2013/04/09 06:00 [entrez]
PHST- 2013/04/09 06:00 [pubmed]
PHST- 2013/06/12 06:00 [medline]
AID - 33/4/1525 [pii]
PST - ppublish
SO  - Anticancer Res. 2013 Apr;33(4):1525-36.