PMID- 23566224
OWN - NLM
STAT- MEDLINE
DCOM- 20140331
LR  - 20220311
IS  - 1460-2709 (Electronic)
IS  - 1369-3786 (Linking)
VI  - 51
IP  - 7
DP  - 2013 Oct
TI  - Early Endosome Antigen 1 (EEA1) decreases in macrophages infected with 
      Paracoccidioides brasiliensis.
PG  - 759-64
LID - 10.3109/13693786.2013.777859 [doi]
AB  - Paracoccidioidomycosis (PCM) is a chronic granulomatous disease caused by the 
      dimorphic fungus Paracoccidioides brasiliensis, endemic in Latin America. P. 
      brasiliensis has been observed in epithelial cells in vivo and in vitro, as well 
      as within the macrophages. The identification of the mechanism by which it 
      survives within the host cell is fertile ground for the discovery of its 
      pathogenesis since this organism has the ability to induce its own endocytosis in 
      epithelial cells and most likely in macrophages. The study of the expression of 
      endocytic proteins pathway and co-localization of microorganisms enable detection 
      of the mechanism by which microorganisms survive within the host cell. The aim of 
      this study was to evaluate the expression of the endocytic protein EEA1 (early 
      endosome antigen 1) in macrophages infected with P. brasiliensis. For detection 
      of EEA1, three different techniques were employed: immunofluorescence, real-time 
      polymerase chain reaction (PCR) and immunoblotting. In the present study, 
      decreased expression of EEA1 as well as the rearrangement of the actin was 
      observed when the fungus was internalized, confirming that the input mechanism of 
      the fungus in macrophages occurs through phagocytosis.
FAU - Voltan, Aline Raquel
AU  - Voltan AR
AD  - * Faculdade de Ciencias Farmaceuticas, UNESP - Univ Estadual Paulista , 
      Araraquara , Sao Paulo.
FAU - Sardi, Janaina De Cassia Orlandi
AU  - Sardi Jde C
FAU - Soares, Christiane Pienna
AU  - Soares CP
FAU - Pelajo Machado, Marcelo
AU  - Pelajo Machado M
FAU - Fusco Almeida, Ana Marisa
AU  - Fusco Almeida AM
FAU - Mendes-Giannini, Maria Jose Soares
AU  - Mendes-Giannini MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130408
PL  - England
TA  - Med Mycol
JT  - Medical mycology
JID - 9815835
RN  - 0 (Actins)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (early endosome antigen 1)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Cell Line
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - *Host-Pathogen Interactions
MH  - Immunoblotting
MH  - Macrophages/*chemistry/*microbiology
MH  - Mice
MH  - Paracoccidioides/*growth & development/*immunology
MH  - Phagocytosis
MH  - Real-Time Polymerase Chain Reaction
MH  - Vesicular Transport Proteins/*analysis/genetics
EDAT- 2013/04/10 06:00
MHDA- 2014/04/01 06:00
CRDT- 2013/04/10 06:00
PHST- 2013/04/10 06:00 [entrez]
PHST- 2013/04/10 06:00 [pubmed]
PHST- 2014/04/01 06:00 [medline]
AID - 10.3109/13693786.2013.777859 [doi]
PST - ppublish
SO  - Med Mycol. 2013 Oct;51(7):759-64. doi: 10.3109/13693786.2013.777859. Epub 2013 
      Apr 8.