PMID- 23566632
OWN - NLM
STAT- MEDLINE
DCOM- 20140514
LR  - 20220408
IS  - 1873-3360 (Electronic)
IS  - 0306-4530 (Linking)
VI  - 38
IP  - 9
DP  - 2013 Sep
TI  - Effects of Lactobacillus helveticus on murine behavior are dependent on diet and 
      genotype and correlate with alterations in the gut microbiome.
PG  - 1738-47
LID - S0306-4530(13)00046-2 [pii]
LID - 10.1016/j.psyneuen.2013.02.008 [doi]
AB  - Modulation of the gut microbiota with diet and probiotic bacteria can restore 
      intestinal homeostasis in inflammatory conditions and alter behavior via the 
      gut-brain axis. The purpose of this study was to determine whether the modulatory 
      effects of probiotics differ depending on diet and mouse genotype. At weaning, 
      wild type (WT) and IL-10 deficient (IL-10(-/-)) 129/SvEv mice were placed on a 
      standard mouse chow or a Western-style diet (fat 33%, refined carbohydrate 
      49%)+/-Lactobacillus helveticus ROO52 (10(9)cfu/d) for 21 days. Animal weight and 
      food eaten were monitored weekly. Intestinal immune function was analysed for 
      cytokine expression using the Meso Scale Discovery platform. Spatial memory and 
      anxiety-like behavior was assessed in a Barnes maze. Terminal restriction 
      fragment length polymorphism (TRFLP) was used to analyze the fecal microbiota. 
      Both WT and IL-10(-/-) mice on a Western diet had increased weight gain along 
      with changes in gut microbiota and cytokine expression and altered anxiety-like 
      behavior. The ability of L. helveticus to modulate these factors was genotype- 
      and diet-dependent. Anxiety-like behavior and memory were negatively affected by 
      Western-style diet depending on inflammatory state, but this change was prevented 
      with L. helveticus administration. However, probiotics alone decreased 
      anxiety-like behavior in WT mice on a chow diet. Mice on the Western diet had 
      decreased inflammation and fecal corticosterone, but these markers did not 
      correlate with changes in behavior. Analysis of bacterial phyla from WT and 
      IL-10(-/-)mice showed discrete clustering of the groups to be associated with 
      both diet and probiotic supplementation, with the diet-induced shift normalized 
      to some degree by L. helveticus. These findings suggest that the type of diet 
      consumed by the host and the presence or absence of active inflammation may 
      significantly alter the ability of probiotics to modulate host physiological 
      function.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Ohland, Christina L
AU  - Ohland CL
AD  - Department of Medicine, University of Alberta, Edmonton, AB, Canada.
FAU - Kish, Lisa
AU  - Kish L
FAU - Bell, Haley
AU  - Bell H
FAU - Thiesen, Aducio
AU  - Thiesen A
FAU - Hotte, Naomi
AU  - Hotte N
FAU - Pankiv, Evelina
AU  - Pankiv E
FAU - Madsen, Karen L
AU  - Madsen KL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130406
PL  - England
TA  - Psychoneuroendocrinology
JT  - Psychoneuroendocrinology
JID - 7612148
RN  - 0 (Cytokines)
RN  - 0 (Fatty Acids)
RN  - 0 (IL10 protein, mouse)
RN  - 130068-27-8 (Interleukin-10)
RN  - V27W9254FZ (Cortisone)
SB  - IM
MH  - *Animal Feed
MH  - Animals
MH  - Anxiety/etiology/*prevention & control
MH  - Colitis/etiology/microbiology/pathology/*prevention & control
MH  - Cortisone/analysis
MH  - Cytokines/metabolism
MH  - Fatty Acids/analysis
MH  - Feces/chemistry
MH  - Gastrointestinal Contents/chemistry
MH  - Genotype
MH  - Hippocampus/pathology
MH  - Inflammation/etiology/*prevention & control
MH  - Interleukin-10/deficiency/genetics
MH  - Intestines/chemistry/*microbiology/pathology
MH  - *Lactobacillus helveticus/physiology
MH  - Maze Learning
MH  - Memory Disorders/etiology/*prevention & control
MH  - Mice
MH  - Microbiota/genetics/*physiology
MH  - Polymorphism, Restriction Fragment Length
MH  - Probiotics/*therapeutic use/toxicity
MH  - Prosencephalon/pathology
MH  - Specific Pathogen-Free Organisms
MH  - Weight Gain
OTO - NOTNLM
OT  - Anxiety
OT  - Behavior
OT  - Colonic inflammation
OT  - Corticosterone
OT  - High fat diet
OT  - Memory
OT  - Microbiota
OT  - Probiotics
EDAT- 2013/04/10 06:00
MHDA- 2014/05/16 06:00
CRDT- 2013/04/10 06:00
PHST- 2012/09/04 00:00 [received]
PHST- 2013/02/08 00:00 [revised]
PHST- 2013/02/08 00:00 [accepted]
PHST- 2013/04/10 06:00 [entrez]
PHST- 2013/04/10 06:00 [pubmed]
PHST- 2014/05/16 06:00 [medline]
AID - S0306-4530(13)00046-2 [pii]
AID - 10.1016/j.psyneuen.2013.02.008 [doi]
PST - ppublish
SO  - Psychoneuroendocrinology. 2013 Sep;38(9):1738-47. doi: 
      10.1016/j.psyneuen.2013.02.008. Epub 2013 Apr 6.