PMID- 23566952
OWN - NLM
STAT- MEDLINE
DCOM- 20130717
LR  - 20211203
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Linking)
VI  - 269
IP  - 3
DP  - 2013 Jun 15
TI  - Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 
      2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro.
PG  - 226-32
LID - S0041-008X(13)00131-2 [pii]
LID - 10.1016/j.taap.2013.03.024 [doi]
AB  - Increasing environmental pollution by carcinogens such as some of persistent 
      organic pollutants (POPs) has prompted growing interest in searching for 
      chemopreventive compounds which are readily obtainable. Sulforaphane (SFN) is 
      isolated from cruciferous vegetables and has the potentials to reduce 
      carcinogenesis through various pathways. In this study, we studied the effects of 
      SFN on CYP1A1 activity and genotoxicity induced by 
      2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that SFN inhibited 
      TCDD-induced CYP1A1 activity in H4IIE cells by directly inhibiting CYP1A1 
      activity, probably through binding to aryl hydrocarbon receptor and/or CYP1A1 
      revealed by molecular docking. However, SFN promoted TCDD-induced DNA damage in 
      yeast cells and reduced the viability of initiated yeast cells. Besides, it is 
      surprising that SFN also failed to reduce genotoxicity induced by other genotoxic 
      reagents which possess different mechanisms to lead to DNA damage. Currently, it 
      is difficult to predict whether SFN has the potentials to reduce the risk of TCDD 
      based on the conflicting observations in the study. Therefore, further studies 
      should be urgent to reveal the function and mechanism of SFN in the stress of 
      such POPs on human health.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Yang, Fangxing
AU  - Yang F
AD  - MOE Key Laboratory of Environmental Remediation and Ecosystem Health, College of 
      Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, 
      China. fxyang@zju.edu.cn
FAU - Zhuang, Shulin
AU  - Zhuang S
FAU - Zhang, Chao
AU  - Zhang C
FAU - Dai, Heping
AU  - Dai H
FAU - Liu, Weiping
AU  - Liu W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130406
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Isothiocyanates)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Sulfoxides)
RN  - 0 (Thiocyanates)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - GA49J4310U (sulforaphane)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cytochrome P-450 CYP1A1/*antagonists & inhibitors
MH  - DNA Damage/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Isothiocyanates
MH  - Mutagenicity Tests
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - Rats
MH  - Saccharomyces cerevisiae/drug effects
MH  - Sulfoxides
MH  - Thiocyanates/pharmacology/*toxicity
EDAT- 2013/04/10 06:00
MHDA- 2013/07/19 06:00
CRDT- 2013/04/10 06:00
PHST- 2012/11/02 00:00 [received]
PHST- 2013/03/11 00:00 [revised]
PHST- 2013/03/18 00:00 [accepted]
PHST- 2013/04/10 06:00 [entrez]
PHST- 2013/04/10 06:00 [pubmed]
PHST- 2013/07/19 06:00 [medline]
AID - S0041-008X(13)00131-2 [pii]
AID - 10.1016/j.taap.2013.03.024 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2013 Jun 15;269(3):226-32. doi: 
      10.1016/j.taap.2013.03.024. Epub 2013 Apr 6.