PMID- 23568215
OWN - NLM
STAT- MEDLINE
DCOM- 20130913
LR  - 20190923
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Linking)
VI  - 23
IP  - 4
DP  - 2013 Apr
TI  - Glyceraldehyde-3-phosphate dehydrogenase, an immunogenic Streptococcus equi ssp. 
      zooepidemicus adhesion protein and protective antigen.
PG  - 579-85
AB  - Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is an 
      important pathogen associated with opportunistic infections of a wide range of 
      species, including pigs and humans. The absence of a suitable vaccine makes it 
      difficult to control SEZ infection. Glyceraldehyde-3-phosphate dehydrogenase 
      (GAPDH) has been previously identified as an immunogenic protein using 
      immunoproteomic techniques. In the present study, we confirmed that the sequence 
      of GAPDH was highly conserved with other Streptococcus spp. The purified 
      recombinant GAPDH could elicit a significant humoral antibody response in mice 
      and confer significant protection against challenge with a lethal dose of SEZ. 
      GAPDH could adhere to the Hep-2 cells, confirmed by flow cytometry, and inhibit 
      adherence of SEZ to Hep-2 cells in an adherence inhibition assay. In addition, 
      real-time PCR demonstrated that GAPDH was induced in vivo following infection of 
      mice with SEZ. These suggest that GAPDH could play an important role in the 
      pathogenesis of SEZ infection and could be a target for vaccination against SEZ.
FAU - Fu, Qiang
AU  - Fu Q
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou 510006, PR China.
FAU - Wei, Zigong
AU  - Wei Z
FAU - Liu, Xiaohong
AU  - Liu X
FAU - Xiao, Pingping
AU  - Xiao P
FAU - Lu, Zhaohui
AU  - Lu Z
FAU - Chen, Yaosheng
AU  - Chen Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (Adhesins, Bacterial)
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, Synthetic)
RN  - EC 1.2.1.9 (Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+))
SB  - IM
MH  - Adhesins, Bacterial/genetics/*immunology/isolation & purification/metabolism
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - Bacterial Adhesion
MH  - Bacterial Vaccines/administration & dosage/genetics/*immunology/isolation & 
      purification
MH  - Cell Line
MH  - Disease Models, Animal
MH  - Gene Expression Profiling
MH  - Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)/genetics/*immunology/isolation & 
      purification/metabolism
MH  - Hepatocytes/microbiology
MH  - Humans
MH  - Mice
MH  - Real-Time Polymerase Chain Reaction
MH  - Recombinant Proteins/genetics/immunology/isolation & purification/metabolism
MH  - Streptococcal Infections/immunology/*prevention & control
MH  - Streptococcus equi/*enzymology/*immunology
MH  - Survival Analysis
MH  - Vaccines, Synthetic/administration & dosage/genetics/immunology/isolation & 
      purification
EDAT- 2013/04/10 06:00
MHDA- 2013/09/14 06:00
CRDT- 2013/04/10 06:00
PHST- 2013/04/10 06:00 [entrez]
PHST- 2013/04/10 06:00 [pubmed]
PHST- 2013/09/14 06:00 [medline]
AID - JMB023-04-20 [pii]
AID - 10.4014/jmb.1209.09037 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2013 Apr;23(4):579-85. doi: 10.4014/jmb.1209.09037.