PMID- 23568233
OWN - NLM
STAT- MEDLINE
DCOM- 20131030
LR  - 20240109
IS  - 1678-2674 (Electronic)
IS  - 0102-8650 (Linking)
VI  - 28
IP  - 4
DP  - 2013 Apr
TI  - Long term evaluation of morphometric and ultrastructural changes of testes of 
      alloxan-induced diabetic rats.
PG  - 256-65
LID - S0102-86502013000400005 [pii]
AB  - PURPOSE: To evaluate in a long term the morphometric and ultrastructural changes 
      in seminiferous tubules (ST) of normal and diabetic rats, and to correlate any 
      changes with animal age and diabetes duration. METHODS: Sixty male Wistar rats, 
      three months-old, were randomly divided into two groups: 30 non-diabetic controls 
      (N) and 30 alloxan untreated diabetic (D). After one, six and 12 months of 
      follow-up or diabetes induction rats were sacrificed and the testes examined. 
      Morphometric measures of the ST were performed by digital imaging analysis. ST 
      ultrastructure was analyzed by transmission electron microscopy. RESULTS: 
      Sustained hyperglycemic state was observed in all diabetic rats throughout the 
      study. Serum testosterone was also significantly decreased in these animals. The 
      diameter, total area, epithelium area and epithelium thickness of ST were lower 
      and tubular density was higher in diabetic animals. Diabetic rats also showed 
      ultrastructural changes compromising the whole testis including germ-, Sertoli-, 
      and Leydig cells, and also the mithocondria and cellular nuclei. Most frequent of 
      these consisted of vacuolization and/or accumulation of lipid droplets and 
      electron dense dark material in cell cytoplasm and/or in membranes, cellular 
      degeneration, and apoptosis. Non-diabetic control rats also showed testicular 
      lesions that resemble to the diabetic lesions, although much less severe and with 
      later onset in life of these animals. CONCLUSION: Histopathological changes 
      observed in testes of normal and diabetic rats are closely related to the animal 
      age and/or duration of the hyperglycemic state, being progressively more severe 
      in animals sacrificed belatedly. These changes may play an important role in male 
      infertility observed in diabetic subjects.
FAU - Trindade, Amelia Arcangela Teixeira
AU  - Trindade AA
AD  - Department of Pediatrics, Faculty of Medicine, Brasilia University, Brasilia, DF, 
      Brazil.
FAU - Simoes, Antonio Caetano Pereira
AU  - Simoes AC
FAU - Silva, Reinaldo Jose
AU  - Silva RJ
FAU - Macedo, Celia Sperandeo
AU  - Macedo CS
FAU - Spadella, Cesar Tadeu
AU  - Spadella CT
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Brazil
TA  - Acta Cir Bras
JT  - Acta cirurgica brasileira
JID - 9103983
RN  - 0 (Blood Glucose)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Insulin)
RN  - 3XMK78S47O (Testosterone)
RN  - 6SW5YHA5NG (Alloxan)
SB  - IM
MH  - Alloxan
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Diabetes Mellitus, Experimental/*pathology/physiopathology
MH  - Glycated Hemoglobin/analysis
MH  - Infertility, Male/metabolism
MH  - Insulin/blood
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Testis/*pathology/ultrastructure
MH  - Testosterone/blood
MH  - Time Factors
EDAT- 2013/04/10 06:00
MHDA- 2013/10/31 06:00
CRDT- 2013/04/10 06:00
PHST- 2012/12/11 00:00 [received]
PHST- 2013/03/14 00:00 [accepted]
PHST- 2013/04/10 06:00 [entrez]
PHST- 2013/04/10 06:00 [pubmed]
PHST- 2013/10/31 06:00 [medline]
AID - S0102-86502013000400005 [pii]
AID - 10.1590/s0102-86502013000400005 [doi]
PST - ppublish
SO  - Acta Cir Bras. 2013 Apr;28(4):256-65. doi: 10.1590/s0102-86502013000400005.