PMID- 23568728 OWN - NLM STAT- MEDLINE DCOM- 20130723 LR - 20130409 IS - 1003-9406 (Print) IS - 1003-9406 (Linking) VI - 30 IP - 2 DP - 2013 Apr TI - [Identification of a novel HLA allele HLA-B*55:35]. PG - 168-71 LID - 10.3760/cma.j.issn.1003-9406.2013.04.010 [doi] AB - OBJECTIVE: To identify a novel human leukocyte antigen (HLA) B allele and explore its family heritage. METHODS: A novel HLA allele was suspected upon routine HLA typing using a polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) assay. The sequence was confirmed with DNA sequencing and compared with its closest matching allele, B*55:02. The family was also investigated. RESULTS: An unusual reaction pattern was detected during routine HLA typing. The sequence was confirmed to be a novel HLA-B allele, which differed from the closest matching allele, B*55:02 in 7 nt positions in exon 2. Among the 7 mutations from 6 codons, there were two amino acids changes including 69Glu-->Met and 70Glu-->Ala. CONCLUSION: A novel HLA-B allele has been identified and officially named as B*55:35 by the WHO Nomenclature Committee for Factors of the HLA System (GenBank accession number FJ898284). FAU - Song, Yong-hong AU - Song YH AD - China Marrow Donor Program HLA Typing Laboratory, Blood Center of Shandong Province, Jinan, P.R. China. FAU - Mao, Yong-xin AU - Mao YX FAU - Dong, Kui AU - Dong K FAU - Chen, Xiao-jian AU - Chen XJ FAU - Gu, Yan-xia AU - Gu YX FAU - Zhu, Chuan-fu AU - Zhu CF LA - chi SI - GENBANK/FJ898284 PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Zhonghua Yi Xue Yi Chuan Xue Za Zhi JT - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics JID - 9425197 RN - 0 (HLA-B Antigens) RN - 0 (HLA-B55 antigen) SB - IM MH - *Alleles MH - Base Sequence MH - HLA-B Antigens/*genetics MH - Histocompatibility Testing MH - Humans MH - Molecular Sequence Data MH - Sequence Analysis, DNA EDAT- 2013/04/10 06:00 MHDA- 2013/07/24 06:00 CRDT- 2013/04/10 06:00 PHST- 2013/04/10 06:00 [entrez] PHST- 2013/04/10 06:00 [pubmed] PHST- 2013/07/24 06:00 [medline] AID - 940630040 [pii] AID - 10.3760/cma.j.issn.1003-9406.2013.04.010 [doi] PST - ppublish SO - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2013 Apr;30(2):168-71. doi: 10.3760/cma.j.issn.1003-9406.2013.04.010.