PMID- 23569327
OWN - NLM
STAT- MEDLINE
DCOM- 20130703
LR  - 20211021
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 210
IP  - 5
DP  - 2013 May 6
TI  - Similar antigen cross-presentation capacity and phagocytic functions in all 
      freshly isolated human lymphoid organ-resident dendritic cells.
PG  - 1035-47
LID - 10.1084/jem.20121103 [doi]
AB  - Dendritic cells (DCs) represent a heterogeneous population of antigen-presenting 
      cells that initiate and orient immune responses in secondary lymphoid organs. In 
      mice, lymphoid organ-resident CD8(+) DCs are specialized at cross-presentation 
      and have developed specific adaptations of their endocytic pathway (high pH, low 
      degradation, and high export to the cytosol). In humans, blood BDCA3(+) DCs were 
      recently shown to be the homologues of mouse CD8(+) DCs. They were also proposed 
      to cross-present antigens more efficiently than other blood DC subsets after in 
      vitro activation, suggesting that in humans cross-presentation is restricted to 
      certain DC subsets. The DCs that cross-present antigen physiologically, however, 
      are the ones present in lymphoid organs. Here, we show that freshly isolated 
      tonsil-resident BDCA1(+) DCs, BDCA3(+) DCs, and pDCs all cross-present soluble 
      antigen efficiently, as compared to macrophages, in the absence of activation. In 
      addition, BDCA1(+) and BDCA3(+) DCs display similar phagosomal pH and similar 
      production of reactive oxygen species in their phagosomes. All three DC subsets, 
      in contrast to macrophages, also efficiently export internalized proteins to the 
      cytosol. We conclude that all freshly isolated lymphoid organ-resident human DCs, 
      but not macrophages, display high intrinsic cross-presentation capacity.
FAU - Segura, Elodie
AU  - Segura E
AD  - Institut National de la Sante et de la Recherche Medicale, U932, 75005 Paris, 
      France.
FAU - Durand, Melanie
AU  - Durand M
FAU - Amigorena, Sebastian
AU  - Amigorena S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130408
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, CD1)
RN  - 0 (Ligands)
RN  - 0 (MART-1 Antigen)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Antigens, CD1/immunology
MH  - Cell Separation
MH  - Cross-Priming/*immunology
MH  - Cytosol/metabolism
MH  - Dendritic Cells/*cytology/*immunology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Ligands
MH  - Lymphoid Tissue/*cytology
MH  - MART-1 Antigen/immunology
MH  - Mice
MH  - Necrosis
MH  - Palatine Tonsil/cytology
MH  - Phagocytes/*immunology
MH  - Phagosomes/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Solubility
MH  - Toll-Like Receptors/immunology
PMC - PMC3646495
EDAT- 2013/04/10 06:00
MHDA- 2013/07/05 06:00
PMCR- 2013/11/06
CRDT- 2013/04/10 06:00
PHST- 2013/04/10 06:00 [entrez]
PHST- 2013/04/10 06:00 [pubmed]
PHST- 2013/07/05 06:00 [medline]
PHST- 2013/11/06 00:00 [pmc-release]
AID - jem.20121103 [pii]
AID - 20121103 [pii]
AID - 10.1084/jem.20121103 [doi]
PST - ppublish
SO  - J Exp Med. 2013 May 6;210(5):1035-47. doi: 10.1084/jem.20121103. Epub 2013 Apr 8.