PMID- 23573982
OWN - NLM
STAT- MEDLINE
DCOM- 20140303
LR  - 20220409
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 75
IP  - 1
DP  - 2013 Jul
TI  - Copy number variation in potato - an asexually propagated autotetraploid species.
PG  - 80-89
LID - 10.1111/tpj.12200 [doi]
AB  - Copy number variation (CNV) has been revealed as a significant contributor to the 
      genetic variation in humans. Although CNV has been reported in several model 
      animal and plant species, the presence of CNV and its biological impact in 
      polyploid species has not yet been documented. We conducted a fluorescence in 
      situ hybridization (FISH)-based CNV survey in potato, a vegetatively propagated 
      autotetraploid species (2n = 4x = 48). We conducted FISH analysis using 18 
      randomly selected potato bacterial artificial chromosome (BAC) clones in a set of 
      16 potato cultivars with diverse breeding backgrounds. Six BACs (33%) with insert 
      sizes of 137-145 kb were found to be associated with large CNV events detectable 
      at the cytological level. We demonstrate that the large CNVs associated with two 
      specific BACs (RH102I10 and RH83C08) were widespread among potato cultivars 
      developed in North America and Europe. We measured the transcript abundance of 
      four genes associated with the CNV spanned by BAC RH102I10. All four genes 
      displayed a dosage effect in transcription. Although potato is vegetatively 
      propagated, we observed that female gametes lacking the RH102I10-associated CNV 
      were inferior to those with at least one copy of this CNV, indicating that the 
      RH102I10-associated CNV can impact on the growth and development of the potato 
      plants. Our results show that CNV is highly abundant in the potato genome and may 
      play a significant role in genetic variation of this important food crop.
CI  - (c) 2013 The Authors The Plant Journal (c) 2013 John Wiley & Sons Ltd.
FAU - Iovene, Marina
AU  - Iovene M
AD  - Department of Horticulture, University of Wisconsin-Madison, Madison, WI, 53706, 
      USA.
AD  - CNR-Institute of Plant Genetics, Bari, 70126, Italy.
FAU - Zhang, Tao
AU  - Zhang T
AD  - Department of Horticulture, University of Wisconsin-Madison, Madison, WI, 53706, 
      USA.
FAU - Lou, Qunfeng
AU  - Lou Q
AD  - Department of Horticulture, University of Wisconsin-Madison, Madison, WI, 53706, 
      USA.
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 
      Agricultural University, Jiangsu, Nanjing, 210095, People's Republic of China.
FAU - Buell, C Robin
AU  - Buell CR
AD  - Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, 
      USA.
FAU - Jiang, Jiming
AU  - Jiang J
AD  - Department of Horticulture, University of Wisconsin-Madison, Madison, WI, 53706, 
      USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130513
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Breeding
MH  - Chromosomes, Artificial, Bacterial
MH  - *DNA Copy Number Variations
MH  - DNA, Plant/genetics
MH  - Gene Dosage
MH  - *Genetic Variation
MH  - Genome, Plant/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Reproduction, Asexual
MH  - Solanum tuberosum/cytology/*genetics/growth & development/physiology
MH  - Species Specificity
MH  - Tetraploidy
MH  - Transcription, Genetic
EDAT- 2013/04/12 06:00
MHDA- 2014/03/04 06:00
CRDT- 2013/04/12 06:00
PHST- 2013/01/05 00:00 [received]
PHST- 2013/03/29 00:00 [revised]
PHST- 2013/04/07 00:00 [accepted]
PHST- 2013/04/12 06:00 [entrez]
PHST- 2013/04/12 06:00 [pubmed]
PHST- 2014/03/04 06:00 [medline]
AID - 10.1111/tpj.12200 [doi]
PST - ppublish
SO  - Plant J. 2013 Jul;75(1):80-89. doi: 10.1111/tpj.12200. Epub 2013 May 13.