PMID- 23582132
OWN - NLM
STAT- MEDLINE
DCOM- 20131031
LR  - 20181202
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 92
DP  - 2013 Jun
TI  - Transcriptional profiling in burbot (Lota lota) from Lake Mjosa--a Norwegian Lake 
      contaminated by several organic pollutants.
PG  - 94-103
LID - S0147-6513(13)00076-6 [pii]
LID - 10.1016/j.ecoenv.2013.02.019 [doi]
AB  - Lake Mjosa is the largest freshwater repository in Norway, receiving runoff from 
      a wide surrounding region of urban country. As a result of industrial activity, 
      large quantities of persistent organic pollutants (POPs) have been discharged 
      into Lake Mjosa during the last century. The levels of PCBs, DDTs and PBDEs in 
      burbot from Lake Mjosa (study population) exceed the corresponding levels in 
      burbot from Lake Losna (reference) by a factor of 3, 6 and 113, respectively. We 
      used shotgun and suppression subtraction hybridization (SSH) cDNA libraries 
      followed by 454 FLX sequencing (957 303 reads sequenced in total) and RT-qPCR to 
      study the effects of POPs in burbot from Lake Mjosa. The gene list of putatively 
      higher or lower expressed genes in liver of burbot from Lake Mjosa compared to 
      liver of burbot from Lake Losna, generated from the SSH cDNA libraries, suggest 
      that mechanisms associated with drug metabolism and oxidative stress are enriched 
      in burbot from Lake Mjosa (Ingenuity Pathway Analysis (IPA) top networks). 
      According to the IPA analyses, the top toxicity list ranking was "LXR/RXR 
      activation", "Negative/Positive acute phase response proteins", 
      "LPS/IL-1-mediated inhibition of RXR function" and "FXR/RXR activation". 
      Functional analyses further identified PPAR, HNF4A, dexamethasone and beta-estradiol 
      as potential upstream key regulator factors. Overall, the study shows that SSH 
      cDNA libraries coupled to next-generation sequencing (RNA-Seq) may be a valuable 
      supplement or alternative to microarray technology in toxicogenomic discovery of 
      environmental samples.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Olsvik, Pal A
AU  - Olsvik PA
AD  - National Institute of Nutrition and Seafood Research, Nordnesboder 1-2, N-5005 
      Bergen, Norway. pal.olsvik@nifes.no
FAU - Berg, Vidar
AU  - Berg V
FAU - Lyche, Jan L
AU  - Lyche JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130410
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Interleukin-1)
RN  - 0 (Water Pollutants, Chemical)
RN  - 4TI98Z838E (Estradiol)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
EIN - Ecotoxicol Environ Saf. 2015 Apr;114:365
MH  - Animals
MH  - Environmental Monitoring
MH  - Estradiol/genetics/metabolism
MH  - Female
MH  - Gadiformes/*metabolism
MH  - Gene Expression
MH  - Halogenated Diphenyl Ethers/analysis/metabolism/toxicity
MH  - Interleukin-1/genetics/metabolism
MH  - Lakes/chemistry
MH  - Liver/drug effects/metabolism
MH  - Male
MH  - Norway
MH  - Polychlorinated Biphenyls/analysis/metabolism/toxicity
MH  - Water Pollutants, Chemical/analysis/*metabolism/toxicity
EDAT- 2013/04/16 06:00
MHDA- 2013/11/01 06:00
CRDT- 2013/04/16 06:00
PHST- 2012/12/04 00:00 [received]
PHST- 2013/02/18 00:00 [revised]
PHST- 2013/02/28 00:00 [accepted]
PHST- 2013/04/16 06:00 [entrez]
PHST- 2013/04/16 06:00 [pubmed]
PHST- 2013/11/01 06:00 [medline]
AID - S0147-6513(13)00076-6 [pii]
AID - 10.1016/j.ecoenv.2013.02.019 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2013 Jun;92:94-103. doi: 10.1016/j.ecoenv.2013.02.019. 
      Epub 2013 Apr 10.