PMID- 23583594
OWN - NLM
STAT- MEDLINE
DCOM- 20130906
LR  - 20130506
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 543
DP  - 2013 May 24
TI  - Injection of neural progenitor cells attenuates decrease in level of connexin 43 
      in brain capillaries after cerebral ischemia.
PG  - 152-6
LID - S0304-3940(13)00296-6 [pii]
LID - 10.1016/j.neulet.2013.03.053 [doi]
AB  - Although functional disruption of the cerebrovasculature, which is called the 
      "neurovascular unit (NVU)", may lead to amplification of ischemia-induced injury, 
      changes in the gap junctional proteins within the NVU and their 
      pathophysiological roles after brain injury remain controversial. We previously 
      demonstrated that the intravenous injection of neural progenitor cells (NPCs) 
      have therapeutic potential for improving the spatial learning dysfunction and 
      depression-like behaviors observed after cerebral ischemia. In this study, we 
      investigated whether severe cerebral ischemia would alter the expression of gap 
      junctional proteins in isolated brain capillaries and examined the effect of 
      intravenous injection of NPCs on the levels of these proteins. Cerebral ischemia 
      induced a sustained decrease in the level of the gap junctional protein connexin 
      43 (Cx43) in the isolated brain capillaries, whereas the level of aquaporin 4 
      (AQP-4) was transiently increased. The injection of NPCs increased the level of 
      Cx43 compared that of vehicle in the microsphere embolism (ME) rats, suggesting 
      this decrease to be a possible mechanism for disruption of the 
      astrocyte-endothelial cell interface within the NVU without causing any changes 
      in the level of AQP-4 and N-cadherin. We also demonstrated that some of the 
      intravenously injected NPCs migrated into the blood vessels in the peri-infarct 
      area. These results suggest that the intravenous injection of the NPCs would 
      remodel the NVU after severe cerebral ischemia, which remodeling might be 
      associated with functional improvement following the NPC injection.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Moriyama, Yoshiyuki
AU  - Moriyama Y
AD  - Department of Molecular and Cellular Pharmacology, Tokyo University of Pharmacy 
      and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
FAU - Takagi, Norio
AU  - Takagi N
FAU - Itokawa, Chisa
AU  - Itokawa C
FAU - Tanonaka, Kouichi
AU  - Tanonaka K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130409
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Aquaporin 4)
RN  - 0 (Cadherins)
RN  - 0 (Connexin 43)
SB  - IM
MH  - Animals
MH  - Aquaporin 4/pharmacology
MH  - Astrocytes/metabolism
MH  - Brain/blood supply/*metabolism
MH  - Brain Ischemia/*metabolism/pathology/physiopathology
MH  - Cadherins/metabolism
MH  - Capillaries/*metabolism
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Connexin 43/*metabolism
MH  - Endothelial Cells/metabolism
MH  - Endothelium, Vascular/metabolism
MH  - Neural Stem Cells/pathology/physiology/*transplantation
MH  - Rats
MH  - Rats, Transgenic
MH  - Rats, Wistar
EDAT- 2013/04/16 06:00
MHDA- 2013/09/07 06:00
CRDT- 2013/04/16 06:00
PHST- 2013/02/01 00:00 [received]
PHST- 2013/03/05 00:00 [revised]
PHST- 2013/03/21 00:00 [accepted]
PHST- 2013/04/16 06:00 [entrez]
PHST- 2013/04/16 06:00 [pubmed]
PHST- 2013/09/07 06:00 [medline]
AID - S0304-3940(13)00296-6 [pii]
AID - 10.1016/j.neulet.2013.03.053 [doi]
PST - ppublish
SO  - Neurosci Lett. 2013 May 24;543:152-6. doi: 10.1016/j.neulet.2013.03.053. Epub 
      2013 Apr 9.