PMID- 23588198
OWN - NLM
STAT- MEDLINE
DCOM- 20140108
LR  - 20200106
IS  - 1873-474X (Electronic)
IS  - 0736-5748 (Linking)
VI  - 31
IP  - 5
DP  - 2013 Aug
TI  - Binge alcohol-induced alterations in BDNF and GDNF expression in central extended 
      amygdala and pyriform cortex on infant rats.
PG  - 287-96
LID - S0736-5748(13)00049-X [pii]
LID - 10.1016/j.ijdevneu.2013.04.002 [doi]
AB  - Mothers who consume alcohol during pregnancy may cause a neurotoxic syndrome 
      termed fetal alcohol spectrum disorder (FASD) in the offspring, which includes 
      cognitive deficits and emotional/social disturbances. These alterations are 
      thought to be caused, at least in part, by alcohol-induced imbalance in 
      neurotrophic factor levels, which are critically involved in normal 
      neurodevelopment. Our goal was to study whether brain-derived neurotrophic factor 
      (BDNF) and glial-derived neurotrophic factor (GDNF) expression were affected by 
      alcohol in central extended amygdala (CEXA) and pyriform cortex (Pyr), structures 
      strongly involved in emotional/social behaviors. Further, we evaluated how these 
      changes could be related to blood and brain alcohol concentrations. Postnatal day 
      (PND) pups at 7, 15 and 20-days old were administered alcohol (2.5g/kg s.c. at 0 
      and 2h) or saline. Immunohistochemistry was used to detect the expression of BDNF 
      and GDNF at 2, 12 and 24h after drug administration. Also, gas chromatography was 
      bused to measure blood alcohol levels (BALs) and brain alcohol levels (BrALs) at 
      each hour, from 2 to 8h after the second alcohol administration. Results showed: 
      (1) alcohol-induced enhancement of BDNF positive cells on PND 7 and 20, a 
      decrease on PND 15 in the CEXA, and no changes in the Pyr on PND 7 and 20, but a 
      diminished on PND 15; (2) GDNF positive cells rise after alcohol administration 
      for the three ages in the CEXA and Pyr except on PND 15, where there was a 
      decline; and (3) pharmacokinetics analysis demonstrated age-related differences 
      showing equal BALs on PND 7 and 20 but higher BALs on PND 15. In contrast, BrALs 
      were higher on PND 7 than 15 and 20. Hence, BALs may not be predictive of BrALs 
      in postnatal rats. Furthermore, we did not find a relationship between age in 
      pharmacokinetic differences and neurotrophins response. In conclusion, the CEXA 
      and Pyr are brain structures sensitive to alcohol-induced imbalance in 
      neurotrophic factors expression; and BALs are not a mirror of BrALs.
CI  - Copyright (c) 2013 ISDN. Published by Elsevier Ltd. All rights reserved.
FAU - Balaszczuk, Veronica
AU  - Balaszczuk V
AD  - Instituto de Investigacion Medica Mercedes y Martin Ferreyra, Friuli 2434, 5016 
      Cordoba, Argentina. vbalaszczuk@psyche.unc.edu.ar
FAU - Bender, Crhistian
AU  - Bender C
FAU - Pereno, German
AU  - Pereno G
FAU - Beltramino, Carlos A
AU  - Beltramino CA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130412
PL  - United States
TA  - Int J Dev Neurosci
JT  - International journal of developmental neuroscience : the official journal of the 
      International Society for Developmental Neuroscience
JID - 8401784
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Aging/drug effects/*metabolism
MH  - Amygdala/drug effects/*metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Binge Drinking
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Ethanol/*blood/*poisoning
MH  - Glial Cell Line-Derived Neurotrophic Factor/*metabolism
MH  - Male
MH  - Metabolic Clearance Rate
MH  - Olfactory Pathways/drug effects/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Distribution
EDAT- 2013/04/17 06:00
MHDA- 2014/01/09 06:00
CRDT- 2013/04/17 06:00
PHST- 2012/09/26 00:00 [received]
PHST- 2013/03/21 00:00 [revised]
PHST- 2013/04/05 00:00 [accepted]
PHST- 2013/04/17 06:00 [entrez]
PHST- 2013/04/17 06:00 [pubmed]
PHST- 2014/01/09 06:00 [medline]
AID - S0736-5748(13)00049-X [pii]
AID - 10.1016/j.ijdevneu.2013.04.002 [doi]
PST - ppublish
SO  - Int J Dev Neurosci. 2013 Aug;31(5):287-96. doi: 10.1016/j.ijdevneu.2013.04.002. 
      Epub 2013 Apr 12.