PMID- 23588808
OWN - NLM
STAT- MEDLINE
DCOM- 20130916
LR  - 20211021
IS  - 1560-2281 (Electronic)
IS  - 1083-3668 (Print)
IS  - 1083-3668 (Linking)
VI  - 18
IP  - 4
DP  - 2013 Apr
TI  - Optical coherence tomography for assessment of microbicide safety in a small 
      animal model.
PG  - 046010
LID - 10.1117/1.JBO.18.4.046010 [doi]
LID - 046010
AB  - Sensitive imaging techniques for small animals are needed to assess drug toxicity 
      in preclinical studies. Optical coherence tomography (OCT) provides a noninvasive 
      tool for high-resolution, depth-resolved visualization of drug-induced changes in 
      tissue morphology. In a mouse model, we utilize OCT to assess vaginal tissue 
      integrity following the application of topical microbicides (drugs used to 
      prevent infection). Mice are challenged with herpes simplex virus-2 (HSV-2) to 
      determine the correlation of tissue damage as quantified by OCT to increased 
      susceptibility. The microbicide benzalkonium chloride (BZK) (0.02, 0.2, or 2%) or 
      phosphate buffered saline control is administered intravaginally. In vivo OCT 
      imaging and collection of tissue samples are performed after treatment. A 
      quantitative OCT scoring system is applied to assess epithelial damage, and the 
      results are compared with those of histology. A separate group of mice are 
      treated similarly then challenged with HSV-2. Epithelial morphology quantified 
      noninvasively by OCT and histology are dose-dependent (p<0.0001). The OCT scoring 
      system detected a significant increase in epithelial damage with increasing BZK 
      concentration (p<0.0001). These results paralleled an increase in HSV-2 
      susceptibility (p<0.005). OCT can be used as a noninvasive tool to assess topical 
      drug toxicity in a small animal model with potential to predict increased 
      susceptibility to vaginal infection.
FAU - Bell, Brent A
AU  - Bell BA
AD  - University of Texas Medical Branch, Center for Biomedical Engineering, 301 
      University Boulevard, Galveston, Texas 77555-1156, USA.
FAU - Vincent, Kathleen L
AU  - Vincent KL
FAU - Bourne, Nigel
AU  - Bourne N
FAU - Vargas, Gracie
AU  - Vargas G
FAU - Motamedi, Massoud
AU  - Motamedi M
LA  - eng
GR  - R21 AI076062/AI/NIAID NIH HHS/United States
GR  - R33AI07606205/AI/NIAID NIH HHS/United States
GR  - R33 AI076062/AI/NIAID NIH HHS/United States
GR  - N01HD53407/HD/NICHD NIH HHS/United States
GR  - N01 HD53407/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Biomed Opt
JT  - Journal of biomedical optics
JID - 9605853
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Benzalkonium Compounds)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents/*toxicity
MH  - Benzalkonium Compounds/toxicity
MH  - Disease Models, Animal
MH  - Disease Susceptibility
MH  - Female
MH  - Herpes Genitalis/microbiology
MH  - Herpesvirus 2, Human/pathogenicity
MH  - Histocytochemistry
MH  - Mice
MH  - Tomography, Optical Coherence/*methods
MH  - Toxicity Tests/*methods
MH  - Vagina/chemistry/drug effects/pathology/virology
PMC - PMC3626380
EDAT- 2013/04/17 06:00
MHDA- 2013/09/17 06:00
PMCR- 2014/04/15
CRDT- 2013/04/17 06:00
PHST- 2013/04/17 06:00 [entrez]
PHST- 2013/04/17 06:00 [pubmed]
PHST- 2013/09/17 06:00 [medline]
PHST- 2014/04/15 00:00 [pmc-release]
AID - 1679048 [pii]
AID - 12810R [pii]
AID - 10.1117/1.JBO.18.4.046010 [doi]
PST - ppublish
SO  - J Biomed Opt. 2013 Apr;18(4):046010. doi: 10.1117/1.JBO.18.4.046010.