PMID- 23592138
OWN - NLM
STAT- MEDLINE
DCOM- 20140911
LR  - 20211021
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 33
IP  - 2
DP  - 2013 Apr
TI  - Inhibitory effects of high glucose/insulin environment on osteoclast formation 
      and resorption in vitro.
PG  - 244-249
LID - 10.1007/s11596-013-1105-z [doi]
AB  - Patients with type 2 diabetes mellitus (T2DM) exhibit hyperglycemia and 
      hyperinsulinemia and increased risk of fracture at early stage, but they were 
      found to have normal or even enhanced bone mineral density (BMD). This study was 
      aimed to examine the molecular mechanisms governing changes in bone structure and 
      integrity under both hyperglycemic and hyperinsulinemic conditions. Monocytes 
      were isolated from the bone marrow of the C57BL/6 mice, induced to differentiate 
      into osteoclasts by receptor activator of nuclear factor kappa-B ligand (RANKL) 
      and macrophage colony-stimulating factor (M-CSF) and exposed to high glucose 
      (33.6 mmol/L), high insulin (1 mumol/L), or a combination of high glucose/high 
      insulin (33.6 mmol/L glucose and 1 mumol/L insulin). Cells cultured in alpha-MEM alone 
      served as control. After four days of incubation, the cells were harvested and 
      stained for tartrate resistant acid phosphatase (TRAP). Osteoclast-related genes 
      including RANK, cathepsin K and TRAP were determined by using real-time PCR. The 
      resorptive activity of osteoclasts was measured by using a pit formation assay. 
      Osteoclasts that were derived from monocytes were of multinucleated nature and 
      positive for TRAP, a characteristic marker of osteoclasts. Cell counting showed 
      that the number of osteoclasts was much less in high glucose and high 
      glucose/high insulin groups than in normal glucose and high insulin groups. The 
      expression levels of RANK and cathepsin K were significantly decreased in high 
      glucose, high insulin and high glucose/high insulin groups as compared with 
      normal glucose group, and the TRAP activity was substantially inhibited in high 
      glucose environment. The pit formation assay revealed that the resorptive 
      activity of osteoclasts was obviously decreased in high glucose group and high 
      glucose/high insulin group as compared with normal group. It was concluded that 
      osteoclastogenesis is suppressed under hyperglycemic and hyperinsulinemic 
      conditions, suggesting a disruption of the bone metabolism in diabetic patients.
FAU - Xu, Fei
AU  - Xu F
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Ye, Ya-Ping
AU  - Ye YP
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Dong, Yong-Hui
AU  - Dong YH
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Guo, Feng-Jing
AU  - Guo FJ
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Chen, An-Min
AU  - Chen AM
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Huang, Shi-Long
AU  - Huang SL
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China. doctorhsl@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130417
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Insulin)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Bone Resorption/*metabolism/*pathology
MH  - Cells, Cultured
MH  - Cellular Microenvironment
MH  - Diabetes Mellitus, Type 2/*metabolism/pathology
MH  - Glucose/*metabolism
MH  - Humans
MH  - Insulin/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteoclasts/*metabolism/*pathology
EDAT- 2013/04/18 06:00
MHDA- 2014/09/12 06:00
CRDT- 2013/04/18 06:00
PHST- 2012/10/08 00:00 [received]
PHST- 2013/04/18 06:00 [entrez]
PHST- 2013/04/18 06:00 [pubmed]
PHST- 2014/09/12 06:00 [medline]
AID - 10.1007/s11596-013-1105-z [pii]
AID - 10.1007/s11596-013-1105-z [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2013 Apr;33(2):244-249. doi: 
      10.1007/s11596-013-1105-z. Epub 2013 Apr 17.