PMID- 23592386
OWN - NLM
STAT- MEDLINE
DCOM- 20140529
LR  - 20131024
IS  - 1099-1565 (Electronic)
IS  - 0958-0344 (Linking)
VI  - 24
IP  - 6
DP  - 2013 Nov-Dec
TI  - Analysis of Cleistopholis patens leaf and trunk bark oils using combined GC- 
      flame ionisation detection, GC-retention index, GC-MS and (13) C-NMR.
PG  - 574-80
LID - 10.1002/pca.2435 [doi]
AB  - INTRODUCTION: Germacrenes A-C are secondary metabolites produced by various 
      plants. They are sesquiterpene hydrocarbons bearing the (E,E)-1,5-cyclodecadiene 
      structure known to undergo thermal rearrangement through a [3.3]-sigmatropic 
      reaction. Such a rearrangement was evidenced by comparing the contents of a given 
      germacrene and the corresponding elemene calculated by GC-flame ionisation 
      detection (FID) with the relative intensities of the signals of both molecules in 
      the (13) C-NMR spectrum of the mixture, recorded at room temperature. OBJECTIVE: 
      To develop a protocol to identify and quantify germacrenes A, B and C and in 
      parallel the corresponding elemenes, using a combination of GC-FID and (13) C-NMR 
      and then provide a correct analysis of Cleistopholis patens essential oils. 
      METHODS: The essential oil was submitted to GC-FID, GC-retention index, GC-MS and 
      (13) C-NMR analyses. The relative percentages of every couple of germacrene and 
      elemene measured by GC-FID were summed. Then, the relative ratio of the mean 
      intensities of the signals of the protonated carbons of a given germacrene and 
      the corresponding elemene was calculated. The contents of both compounds were 
      obtained by combining GC-FID and (13) C-NMR data. RESULTS: The true content of 
      germacrene A/beta-elemene, germacrene B/gamma-elemene and germacrene C/delta-elemene in leaf 
      and root oils from C. patens was evaluated by combination of GC-FID and (13) 
      C-NMR data. Correct analysis of the essential oils was provided. CONCLUSION: 
      Combined analysis of essential oil including (13) C-NMR without isolation of the 
      components, appeared really efficient to identify and quantify germacrene isomers 
      and in parallel elemene isomers in essential oils.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - Ouattara, Zana A
AU  - Ouattara ZA
AD  - Universite de Corse-CNRS, UMR 6134 SPE, Equipe Chimie et Biomasse, Route des 
      Sanguinaires, 20000, Ajaccio, France; Laboratoire de Chimie Organique Biologique, 
      UFR-SSMT, Universite Felix Houphouet-Boigny, BPV 34, Abidjan, Cote d'Ivoire.
FAU - Boti, Jean Brice
AU  - Boti JB
FAU - Ahibo, Antoine Coffy
AU  - Ahibo AC
FAU - Casanova, Joseph
AU  - Casanova J
FAU - Tomi, Felix
AU  - Tomi F
FAU - Bighelli, Ange
AU  - Bighelli A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130417
PL  - England
TA  - Phytochem Anal
JT  - Phytochemical analysis : PCA
JID - 9200492
RN  - 0 (Plant Oils)
RN  - 0 (Sesquiterpenes, Germacrane)
SB  - IM
MH  - Annonaceae/*chemistry
MH  - Cote d'Ivoire
MH  - Gas Chromatography-Mass Spectrometry
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Plant Bark/chemistry
MH  - Plant Leaves/chemistry
MH  - Plant Oils/*analysis
MH  - Sesquiterpenes, Germacrane/*analysis/chemistry
OTO - NOTNLM
OT  - 13C-NMR
OT  - Cleistopholis patens
OT  - elemene
OT  - essential oil composition
OT  - germacrene
EDAT- 2013/04/18 06:00
MHDA- 2014/05/30 06:00
CRDT- 2013/04/18 06:00
PHST- 2012/10/31 00:00 [received]
PHST- 2013/02/13 00:00 [revised]
PHST- 2013/02/19 00:00 [accepted]
PHST- 2013/04/18 06:00 [entrez]
PHST- 2013/04/18 06:00 [pubmed]
PHST- 2014/05/30 06:00 [medline]
AID - 10.1002/pca.2435 [doi]
PST - ppublish
SO  - Phytochem Anal. 2013 Nov-Dec;24(6):574-80. doi: 10.1002/pca.2435. Epub 2013 Apr 
      17.