PMID- 23593194
OWN - NLM
STAT- MEDLINE
DCOM- 20131104
LR  - 20240319
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 4
DP  - 2013
TI  - Variation in dietary salt intake induces coordinated dynamics of monocyte subsets 
      and monocyte-platelet aggregates in humans: implications in end organ 
      inflammation.
PG  - e60332
LID - 10.1371/journal.pone.0060332 [doi]
LID - e60332
AB  - BACKGROUND: Monocyte activation and tissue infiltration are quantitatively 
      associated with high-salt intake induced target organ inflammation. We 
      hypothesized that high-salt challenge would induce the expansion of CD14++CD16+ 
      monocytes, one of the three monocyte subsets with a pro-inflammatory phenotype, 
      that is associated with target organ inflammation in humans. 
      METHODOLOGY/PRINCIPAL FINDINGS: A dietary intervention study was performed in 20 
      healthy volunteers, starting with a 3-day usual diet and followed with a 7-day 
      high-salt diet (>/=15 g NaCl/day), and a 7-day low-salt diet (</=5 g NaCl/day). The 
      amounts of three monocyte subsets ("classical" CD14++CD16-, "intermediate" 
      CD14++CD16+ and "non-classical" CD14+CD16++) and their associations with 
      monocyte-platelet aggregates (MPAs) were measured by flow cytometry. Blood oxygen 
      level-dependent magnetic resonance imaging (BOLD-MRI) was used to evaluate renal 
      hypoxia. Switching to a high-salt diet resulted in CD14++ monocyte activation and 
      a rapid expansion of CD14++CD16+ subset and MPAs, with a reciprocal decrease in 
      the percentages of CD14++CD16- and CD14+CD16++ subsets. In vitro study using 
      purified CD14++ monocytes revealed that elevation in extracellular [Na(+)] could 
      lead to CD14++CD16+ expansion via a ROS dependent manner. In addition, high-salt 
      intake was associated with progressive hypoxia in the renal medulla (increased 
      R2* signal) and enhanced urinary monocyte chemoattractant protein-1 (MCP-1) 
      excretion, indicating a temporal and spatial correlation between CD14++CD16+ 
      subset and renal inflammation. The above changes could be completely reversed by 
      a low-salt diet, whereas blood pressure levels remained unchanged during dietary 
      intervention. CONCLUSIONS/SIGNIFICANCE: The present work demonstrates that 
      short-term increases in dietary salt intake could induce the expansion of 
      CD14++CD16+ monocytes, as well as an elevation of MPAs, which might be the 
      underlying cellular basis of high-salt induced end organ inflammation and 
      potential thromboembolic risk. In addition, this process seems largely unrelated 
      to changes in blood pressure levels. This finding provides novel links between 
      dietary salt intake, innate immunity and end organ inflammation.
FAU - Zhou, Xin
AU  - Zhou X
AD  - Institute of Cardiovascular Disease and Heart Center, Pingjin Hospital, Logistics 
      University of Chinese People's Armed Police Forces, Tianjin, China.
FAU - Zhang, Ling
AU  - Zhang L
FAU - Ji, Wen-Jie
AU  - Ji WJ
FAU - Yuan, Fei
AU  - Yuan F
FAU - Guo, Zhao-Zeng
AU  - Guo ZZ
FAU - Pang, Bo
AU  - Pang B
FAU - Luo, Tao
AU  - Luo T
FAU - Liu, Xing
AU  - Liu X
FAU - Zhang, Wen-Cheng
AU  - Zhang WC
FAU - Jiang, Tie-Min
AU  - Jiang TM
FAU - Zhang, Zhuoli
AU  - Zhang Z
FAU - Li, Yu-Ming
AU  - Li YM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130404
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, CD)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Sodium Chloride, Dietary)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Antigens, CD/metabolism
MH  - Blood Chemical Analysis
MH  - Blood Platelets/immunology/*metabolism
MH  - Blood Pressure
MH  - Cell Adhesion
MH  - Cell Communication/immunology
MH  - Creatinine/urine
MH  - *Diet
MH  - Gene Expression Profiling
MH  - Heart Rate
MH  - Humans
MH  - Immunophenotyping
MH  - Inflammation/immunology/metabolism/pathology
MH  - Kidney/immunology/metabolism/pathology
MH  - Magnetic Resonance Imaging
MH  - Monocytes/immunology/*metabolism
MH  - Oxidative Stress
MH  - Oxygen Consumption
MH  - Reactive Oxygen Species/metabolism
MH  - Renal Circulation
MH  - *Sodium Chloride, Dietary
PMC - PMC3617205
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/04/18 06:00
MHDA- 2013/11/05 06:00
PMCR- 2013/04/04
CRDT- 2013/04/18 06:00
PHST- 2012/11/16 00:00 [received]
PHST- 2013/02/25 00:00 [accepted]
PHST- 2013/04/18 06:00 [entrez]
PHST- 2013/04/18 06:00 [pubmed]
PHST- 2013/11/05 06:00 [medline]
PHST- 2013/04/04 00:00 [pmc-release]
AID - PONE-D-12-36260 [pii]
AID - 10.1371/journal.pone.0060332 [doi]
PST - epublish
SO  - PLoS One. 2013 Apr 4;8(4):e60332. doi: 10.1371/journal.pone.0060332. Print 2013.