PMID- 23593564
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130418
LR  - 20211021
IS  - 2164-7844 (Print)
IS  - 2164-7860 (Electronic)
IS  - 2164-7844 (Linking)
VI  - 2
IP  - 2
DP  - 2013 Apr
TI  - Hyperhomocysteinemia-Induced Monocyte Chemoattractant Protein-1 Promoter DNA 
      Methylation by Nuclear Factor-kappaB/DNA Methyltransferase 1 in Apolipoprotein 
      E-Deficient Mice.
PG  - 118-27
LID - 10.1089/biores.2012.0300 [doi]
AB  - Hyperhomocysteinemia is considered to be a significant risk factor in 
      atherosclerosis and plays an important role in it. The purpose of this study was 
      to determine the molecular mechanism of blood monocyte chemoattractant protein-1 
      (MCP-1) promoter DNA hypomethylation in the formation of atherosclerosis induced 
      by hyperhomocysteinemia, and to explore the effect of nuclear factor-kappaB 
      (NF-kappaB)/DNA methyltransferase 1 (DNMT1) in this mechanism. The atherosclerotic 
      effect of MCP-1 in apolipoprotein E-deficient (ApoE(-/-)) and wild-type C57BL/6J 
      mice was evaluated using atherosclerotic lesion area; serum NF-kappaB, MCP-1, and 
      DNMT1 levels; and MCP-1 promoter DNA methylation expression. In vitro, the 
      mechanism responsible for the effect of NF-kappaB/DNMT1 on foam cells was 
      investigated by measuring NF-kappaB and DNMT1 levels to determine whether NF-kappaB/DNMT1 
      had an effect on gene expression. Compared with the control group, 
      atherosclerotic lesions in ApoE(-/-) mice fed a high methionine diet 
      significantly increased, as did the expression of MCP-1. In vitro study showed 
      that pyrrolidine dithiocarbamate treatment down-regulated levels of NF-kappaB and 
      raised DNMT1 concentrations, confirming the effect of NF-kappaB/DNMT1 in the MCP-1 
      promoter DNA methylation process. In conclusion, our results suggest that through 
      NF-kappaB/DNMT1, MCP-1 promoter DNA hypomethylation may play a key role in formation 
      of atherosclerosis under hyperhomocysteinemia.
FAU - Wang, Ju
AU  - Wang J
AD  - Department of Inspection, Ningxia Medical University , Yinchuan, China .
FAU - Jiang, Yideng
AU  - Jiang Y
FAU - Yang, Anning
AU  - Yang A
FAU - Sun, Weiwei
AU  - Sun W
FAU - Ma, Changjian
AU  - Ma C
FAU - Ma, Shengchao
AU  - Ma S
FAU - Gong, Huihui
AU  - Gong H
FAU - Shi, Yingkang
AU  - Shi Y
FAU - Wei, Jun
AU  - Wei J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biores Open Access
JT  - BioResearch open access
JID - 101579333
PMC - PMC3620495
OTO - NOTNLM
OT  - DNA methylation
OT  - atherosclerosis
OT  - hyperhomocysteinemia
OT  - monocyte chemoattractant protein-1
EDAT- 2013/04/18 06:00
MHDA- 2013/04/18 06:01
PMCR- 2013/04/01
CRDT- 2013/04/18 06:00
PHST- 2013/04/18 06:00 [entrez]
PHST- 2013/04/18 06:00 [pubmed]
PHST- 2013/04/18 06:01 [medline]
PHST- 2013/04/01 00:00 [pmc-release]
AID - 10.1089/biores.2012.0300 [pii]
AID - 10.1089/biores.2012.0300 [doi]
PST - ppublish
SO  - Biores Open Access. 2013 Apr;2(2):118-27. doi: 10.1089/biores.2012.0300.