PMID- 23596060
OWN - NLM
STAT- MEDLINE
DCOM- 20160108
LR  - 20150501
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 102
IP  - 1
DP  - 2014 Jan
TI  - The in vitro and in vivo cementogenesis of CaMgSi(2)O(6) bioceramic scaffolds.
PG  - 105-16
LID - 10.1002/jbm.a.34679 [doi]
AB  - The goal of periodontal tissue engineering is to regenerate alveolar bone, root 
      cementum and periodontal ligament. To achieve this goal, bioactive scaffolds play 
      an important role in inducing in vitro osteogenic/cementogenic gene expression of 
      periodontal ligament cells (PDLCs) and in vivo bone/cementum formation. Diopside 
      (DIOP: CaMgSi2O6) ceramics have shown excellent in vitro bioactivity for 
      potential bone repair application. However, there is no study about DIOP porous 
      scaffolds for periodontal tissue engineering. The aim of this study is to prepare 
      DIOP scaffolds and investigate their in vitro and in vivo 
      osteogenesis/cementogenesis for periodontal regeneration application. DIOP 
      scaffolds with highly porous architecture were prepared and beta-tricalcium 
      phosphate (beta-TCP) scaffolds were used for the control. The interaction of DIOP 
      scaffolds with PDLCs was studied by investigating cell attachment, proliferation 
      and ostegenic/cementogenic differentiation of PDLCs. DIOP scaffolds were 
      implanted into the periodontal defects of beagle dogs to evaluate their in vivo 
      osteogenesis/cementogenesis by hematoxylin and eosin (H&E), tartrate-resistant 
      acid phosphatase staining, and immunohistochemistry (type I collagen: Col I; 
      cementum attachment protein) analyses. The results have shown that DIOP scaffolds 
      supported the attachment and proliferation of PDLCs. DIOP scaffolds significantly 
      enhanced osteogenesis/cementogenesis-related gene expression (Col 1, Runx2, 
      transforming growth factor beta 1, and bone morphogenetic protein 2) of PDLCs, 
      compared to beta-TCP scaffolds. The in vivo study showed that DIOP scaffolds induced 
      new bone and cementum regeneration of periodontal tissue defects. The rate of new 
      bone and cementum in DIOP scaffolds is comparable to that in conventional beta-TCP 
      scaffolds. Our results indicated that silicate-based DIOP ceramics could not only 
      be used for bone tissue engineering, but also for periodontal tissue engineering 
      due to their excellent in vitro and in vivo osteogeneis/cementogenesis.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc., a Wiley Company.
FAU - Zhang, Yufeng
AU  - Zhang Y
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST), School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, 
      Wuhan 430079, People's Republic of China; Key Laboratory of Oral Biomedicine 
      Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 
      Luoyu Road, Wuhan 430079, People's Republic of China.
FAU - Li, Shue
AU  - Li S
FAU - Wu, Chengtie
AU  - Wu C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130418
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Calcium Phosphates)
RN  - 0 (beta-tricalcium phosphate)
RN  - 1343-98-2 (Silicic Acid)
RN  - 14483-19-3 (diopside)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/biosynthesis
MH  - Calcium Phosphates/chemistry/pharmacology
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Cementogenesis/*drug effects
MH  - *Ceramics/chemistry/pharmacology
MH  - Dogs
MH  - Gene Expression Regulation/drug effects
MH  - Male
MH  - Periodontal Ligament/cytology/*metabolism
MH  - *Prostheses and Implants
MH  - *Silicic Acid/chemistry/pharmacology
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - bioactive ceramics
OT  - ostegenic/cementogenic differentiation
OT  - periodontal tissue engineering
OT  - scaffolds
EDAT- 2013/04/19 06:00
MHDA- 2016/01/09 06:00
CRDT- 2013/04/19 06:00
PHST- 2012/10/07 00:00 [received]
PHST- 2013/01/23 00:00 [revised]
PHST- 2013/02/21 00:00 [accepted]
PHST- 2013/04/19 06:00 [entrez]
PHST- 2013/04/19 06:00 [pubmed]
PHST- 2016/01/09 06:00 [medline]
AID - 10.1002/jbm.a.34679 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2014 Jan;102(1):105-16. doi: 10.1002/jbm.a.34679. Epub 2013 
      Apr 18.