PMID- 23596791
OWN - NLM
STAT- MEDLINE
DCOM- 20140410
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 33
IP  - 1
DP  - 2013 Jan
TI  - [Comparative proteomics analysis of the ileum of Pi-Yang deficiency model rats].
PG  - 71-5
AB  - OBJECTIVE: Based on proteomics technology, Pi-yang deficiency syndrome (PYDS) 
      correlated differential proteins were screened, thus providing powerful 
      experiment reliance for exploring the essence of PYDS. METHODS: Totally 36 SD 
      rats of SPF grade were randomly divided into the normal control group (n = 16) 
      and the PYDS group (n = 20). The PYDS model rats were induced by improper diet, 
      overstrain, and administration of yang impairing bitter cold herbs. The total 
      proteins of the ileum were separated and extracted from rats in the PYDS group 
      and the normal control group. The differential protein dots were identified using 
      Delyder 2D 6.5 image analysis software by two-dimensional gel electrophoresis 
      (2-DE) technology. The finger print map of corresponding peptide qualities was 
      obtained by applying MALDI TOF/TOF. The differential proteins were identified 
      using Mascot search library. RESULTS: Judged by statistics and fuzzy mathematics, 
      Pi-yang deficiency rat model was successfully established. Eight proteins with 
      differential expressions involving cell skeleton, energy metabolism, and signal 
      transduction, and so on were obtained. Of them, there were 4 up-regulated 
      proteins, i.e., desmin, cytokeratin8 (CK8), pyruvate kinase (PK), and ezrin. Four 
      down-regulated proteins were glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 
      cytokeratin19 (CK19), cytokeratin1 (CK1), and actin. CONCLUSION: The pathogenesis 
      of PYDS might be slowed energy metabolism rate, reduced energy production, 
      changed structure of ileal villin, and weakened absorbing and digestive 
      functions.
FAU - Yu, Man
AU  - Yu M
AD  - Basic Medicine College, Liaoning University of Traditional Chinese Medicine, 
      Shenyang 100847, China.
FAU - Lu, Ling
AU  - Lu L
FAU - Wang, Cai-Xia
AU  - Wang CX
FAU - Jia, Lian-Qun
AU  - Jia LQ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - Female
MH  - Ileum/*metabolism
MH  - Male
MH  - *Medicine, Chinese Traditional
MH  - Proteome/*metabolism
MH  - Proteomics
MH  - Rats
MH  - Yang Deficiency/*diagnosis/*metabolism
EDAT- 2013/04/20 06:00
MHDA- 2014/04/11 06:00
CRDT- 2013/04/20 06:00
PHST- 2013/04/20 06:00 [entrez]
PHST- 2013/04/20 06:00 [pubmed]
PHST- 2014/04/11 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 Jan;33(1):71-5.