PMID- 23596884
OWN - NLM
STAT- MEDLINE
DCOM- 20130628
LR  - 20130419
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 38
IP  - 1
DP  - 2013 Jan
TI  - [Study on in vitro anti-inflammatory activity of total flavonoids from 
      Glycyrrhizae Radix et Rhizoma and its ingredients].
PG  - 99-104
AB  - OBJECTIVE: To examine the anti-inflammatory mechanism of total flavonoids of 
      Glycyrrhizae Radix et Rhizoma (TFGR) and its ingredient on IFN-gamma and 
      LPS-induced macrophage RAW264.7. METHOD: Solvent extraction and macroporous resin 
      enrichment were adopted for preparing ethanol extracts of Glycyrrhizae Radix et 
      Rhizoma, components and total flavonoids. Ultraviolet spectroscopy was used to 
      determine the content of total flavonoids. An IFN-gamma and LPS-induced cell 
      inflammatory model was established. Griess reaction was used for detecting the 
      effect of extracts at all levels and flavonoid monomers on nitrite content in 
      cell culture supernatant. FRAP was used for measuring anti-oxidation capacity. 
      RT-PCR was used for determining the effect of TFGR and isoliquiritigenins on 
      intracellular inducible nitric oxide synthase iNOS, COX-2, IL-6 and PPAR-gamma. 
      Western blot was used for detecting the effect of TFGR and isoliquiritigenins on 
      iNOS, COX-2 and MAPK signal transduction pathways. RESULT: Compared with other 
      extracts, ethyl acetate fractions from Glycyrrhizae Radix et Rhizoma showed the 
      highest inhibition ratio on nitrite content at the same concentration. After 
      being enriched with macroporous resin, TFGR (60. 08% of liquiritin) of ethyl 
      acetate extracts from Glycyrrhizae Radix et Rhizoma showed dose-dependence, and 
      inhibited the nitrite content in cell culture supernatant, which was superior to 
      ethyl acetate extracts, and had the protective effect on post-stimulated cell 
      activity, with a stronger total anti-oxidation than other extracts. TFGR 
      inhibited iNOS, IL-6 mRNA, protein expressions of iNOS, COX-2 and IL-6. 
      Isoliquiritigenin, a flavonoid monomer, could inhibited iNOS, COX-2 gene and 
      protein expression and gene expressions of IL-1beta and IL-6, and 
      upside-regulated gene expression of PPAR-gamma. CONCLUSION: Activity-oriented 
      extraction suggests that ethyl acetate fractions from Glycyrrhizae Radix et 
      Rhizoma is one of components with anti-inflammatory activity. TFGR obtained by 
      enriching the active component showed dose-dependence, and inhibited the nitrite 
      content in cell culture supernatant. The anti-inflammatory effect is partially 
      achieved by regulating ERK signal pathway and inhibiting iNOS and COX-2 gene and 
      protein expressions through extracellular signals of mitogen activated protein 
      kinases (MAPKs). Specifically, isoliquiritigenin may be a component with TFGR 
      anti-inflammatory activity.
FAU - Yang, Xiao-Lu
AU  - Yang XL
AD  - Murad Research Institute for Modernized Chinese Medicine, Shanghai University of 
      Traditional Chinese Medicine, Shanghai 201203, China. luwer1986@126.com
FAU - Liu, Duo
AU  - Liu D
FAU - Bian, Ka
AU  - Bian K
FAU - Zhang, Dan-Dan
AU  - Zhang DD
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Line
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Flavonoids/*pharmacology
MH  - Glycyrrhiza/*chemistry
MH  - Interleukin-1beta/genetics/immunology
MH  - Interleukin-6/genetics/immunology
MH  - Macrophages/drug effects/immunology
MH  - Mice
MH  - NF-kappa B/genetics/immunology
MH  - Rhizome/*chemistry
EDAT- 2013/04/20 06:00
MHDA- 2013/07/03 06:00
CRDT- 2013/04/20 06:00
PHST- 2013/04/20 06:00 [entrez]
PHST- 2013/04/20 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2013 Jan;38(1):99-104.