PMID- 23600852
OWN - NLM
STAT- MEDLINE
DCOM- 20131122
LR  - 20130521
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 85
IP  - 10
DP  - 2013 May 21
TI  - Monitoring metabolic responses of single mitochondria within 
      poly(dimethylsiloxane) wells: study of their endogenous reduced nicotinamide 
      adenine dinucleotide evolution.
PG  - 5146-52
LID - 10.1021/ac400494e [doi]
AB  - It is now demonstrated that mitochondria individually function differently 
      because of specific energetic needs in cell compartments but also because of the 
      genetic heterogeneity within the mitochondrial pool-network of a cell. 
      Consequently, understanding mitochondrial functioning at the single organelle 
      level is of high interest for biomedical research, therefore being a target for 
      analyticians. In this context, we developed easy-to-build platforms of milli- to 
      microwells for fluorescence microscopy of single isolated mitochondria. 
      Poly(dimethylsiloxane) (PDMS) was determined to be an excellent material for 
      mitochondrial deposition and observation of their NADH content. Because of NADH 
      autofluorescence, the metabolic status of each mitochondrion was analyzed 
      following addition of a respiratory substrate (stage 2), ethanol herein, and a 
      respiratory inhibitor (stage 3), Antimycin A. Mean levels of mitochondrial NADH 
      were increased by 32% and 62% under stages 2 and 3, respectively. Statistical 
      studies of NADH value distributions evidenced different types of responses, at 
      least three, to ethanol and Antimycin A within the mitochondrial population. In 
      addition, we showed that mitochondrial ability to generate high levels of NADH, 
      that is its metabolic performance, is not correlated either to the initial 
      energetic state or to the respective size of each mitochondrion.
FAU - Suraniti, Emmanuel
AU  - Suraniti E
AD  - University Bordeaux, ISM, UMR5255, F-33400 Talence, France.
FAU - Vajrala, Venkata Suresh
AU  - Vajrala VS
FAU - Goudeau, Bertrand
AU  - Goudeau B
FAU - Bottari, Serge P
AU  - Bottari SP
FAU - Rigoulet, Michel
AU  - Rigoulet M
FAU - Devin, Anne
AU  - Devin A
FAU - Sojic, Neso
AU  - Sojic N
FAU - Arbault, Stephane
AU  - Arbault S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130507
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0U46U6E8UK (NAD)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Microarray Analysis/*methods
MH  - Mitochondria/*metabolism
MH  - NAD/*metabolism
MH  - Saccharomyces cerevisiae/cytology
MH  - Spectrometry, Fluorescence
EDAT- 2013/04/23 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/04/23 06:00
PHST- 2013/04/23 06:00 [entrez]
PHST- 2013/04/23 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 10.1021/ac400494e [doi]
PST - ppublish
SO  - Anal Chem. 2013 May 21;85(10):5146-52. doi: 10.1021/ac400494e. Epub 2013 May 7.