PMID- 23601004
OWN - NLM
STAT- MEDLINE
DCOM- 20140218
LR  - 20211021
IS  - 1365-2583 (Electronic)
IS  - 0962-1075 (Print)
IS  - 0962-1075 (Linking)
VI  - 22
IP  - 4
DP  - 2013 Aug
TI  - Identification and biochemical characterization of Laodelphax striatellus neutral 
      ceramidase.
PG  - 366-75
LID - 10.1111/imb.12028 [doi]
AB  - Ceramidases are a group of enzymes that catalyse hydrolysis of ceramides to 
      generate fatty acid and sphingosine. In this study, we report the cloning and 
      characterization of the rice small brown planthopper Laodelphax striatellus 
      neutral ceramidase (nCDase), LsnCer. LsnCer was identified by sequencing the 
      transcriptome of L. striatellus and is a protein of 717 amino acids with a 
      predicted molecular weight of 79.3 kDa. Similarly to other known nCDases, the 
      optimum pH for LsnCer is 8.0 and the optimum temperature is 37  degrees C for its in 
      vitro activity. LsnCer activity is inhibited by Zn(2+) significantly and Fe(2+) 
      slightly. LsnCer has broad substrate specificity with a preference for ceramides 
      with a medium acyl-chain or a monounsaturated long acyl-chain. Infection with 
      rice strip virus (RSV) or treatment with insecticides significantly increased 
      LsnCer mRNA expression and its enzymatic activity in L. striatellus. These 
      results suggest that LsnCer is a bona fide nCDase that may have a role in 
      adaption of L. striatellus to environmental stresses.
CI  - (c) 2013 Royal Entomological Society.
FAU - Zhou, Y
AU  - Zhou Y
AD  - State Key Laboratory of Rice Biology, Key laboratory of Agricultural Entomology, 
      the Ministry of Agriculture of China, Hangzhou, Zhejiang, China.
FAU - Lin, X-W
AU  - Lin XW
FAU - Zhang, Y-R
AU  - Zhang YR
FAU - Huang, Y-J
AU  - Huang YJ
FAU - Zhang, C-H
AU  - Zhang CH
FAU - Yang, Q
AU  - Yang Q
FAU - Li, H-Y
AU  - Li HY
FAU - Yuan, J-Q
AU  - Yuan JQ
FAU - Cheng, J-A
AU  - Cheng JA
FAU - Xu, R
AU  - Xu R
FAU - Mao, C
AU  - Mao C
FAU - Zhu, Z-R
AU  - Zhu ZR
LA  - eng
GR  - R01 CA163825/CA/NCI NIH HHS/United States
GR  - R01CA163825/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130419
PL  - England
TA  - Insect Mol Biol
JT  - Insect molecular biology
JID - 9303579
RN  - 0 (Insecticides)
RN  - EC 3.5.1.23 (Ceramidases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Ceramidases/genetics/*metabolism
MH  - Female
MH  - Hemiptera/*enzymology/genetics/virology
MH  - *Host-Pathogen Interactions
MH  - *Insecticides
MH  - Male
MH  - Molecular Sequence Data
MH  - Sequence Analysis, DNA
MH  - Stress, Physiological
MH  - Tenuivirus/*physiology
PMC - PMC3879266
MID - NIHMS540011
OTO - NOTNLM
OT  - LsnCer
OT  - activity
OT  - insecticide
OT  - mRNA
OT  - nCDase
OT  - rice strip virus
EDAT- 2013/04/23 06:00
MHDA- 2014/02/19 06:00
PMCR- 2014/08/01
CRDT- 2013/04/23 06:00
PHST- 2013/04/23 06:00 [entrez]
PHST- 2013/04/23 06:00 [pubmed]
PHST- 2014/02/19 06:00 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - 10.1111/imb.12028 [doi]
PST - ppublish
SO  - Insect Mol Biol. 2013 Aug;22(4):366-75. doi: 10.1111/imb.12028. Epub 2013 Apr 19.