PMID- 23601049
OWN - NLM
STAT- MEDLINE
DCOM- 20140123
LR  - 20191210
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 38
IP  - 2
DP  - 2013 Jul
TI  - Striatal modulation of BDNF expression using microRNA124a-expressing lentiviral 
      vectors impairs ethanol-induced conditioned-place preference and voluntary 
      alcohol consumption.
PG  - 2328-37
LID - 10.1111/ejn.12228 [doi]
AB  - Alcohol abuse is a major health, economic and social concern in modern societies, 
      but the exact molecular mechanisms underlying ethanol addiction remain elusive. 
      Recent findings show that small non-coding microRNA (miRNA) signaling contributes 
      to complex behavioral disorders including drug addiction. However, the role of 
      miRNAs in ethanol-induced conditioned-place preference (CPP) and voluntary 
      alcohol consumption has not yet been directly addressed. Here, we assessed the 
      expression profile of miR124a in the dorsal striatum of rats upon ethanol intake. 
      The results show that miR124a was downregulated in the dorso-lateral striatum 
      (DLS) following alcohol drinking. Then, we identified brain-derived neurotrophic 
      factor (BDNF) as a direct target of miR124a. In fact, BDNF mRNA was upregulated 
      following ethanol drinking. We used lentiviral vector (LV) gene transfer 
      technology to further address the role of miR124a and its direct target BDNF in 
      ethanol-induced CPP and alcohol consumption. Results reveal that stereotaxic 
      injection of LV-miR124a in the DLS enhances ethanol-induced CPP as well as 
      voluntary alcohol consumption in a two-bottle choice drinking paradigm. Moreover, 
      miR124a-silencer (LV-siR124a) as well as LV-BDNF infusion in the DLS attenuates 
      ethanol-induced CPP as well as voluntary alcohol consumption. Importantly, 
      LV-miR124a, LV-siR124a and LV-BDNF have no effect on saccharin and quinine 
      intake. Our findings indicate that striatal miR124a and BDNF signaling have 
      crucial roles in alcohol consumption and ethanol conditioned reward.
CI  - (c) 2013 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.
FAU - Bahi, Amine
AU  - Bahi A
AD  - Department of Anatomy, Tawam Medical Campus, CMHS, United Arab Emirates 
      University, Al Ain, UAE. amine.bahi@uaeu.ac.ae
FAU - Dreyer, Jean-Luc
AU  - Dreyer JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130419
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MIRN124 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Alcohol Drinking/blood/genetics/*metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism/pharmacology
MH  - Conditioning, Psychological/drug effects
MH  - Corpus Striatum/*metabolism
MH  - Ethanol
MH  - Genetic Vectors
MH  - Lentivirus/genetics
MH  - Male
MH  - MicroRNAs/genetics/*metabolism/pharmacology
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - BDNF
OT  - conditioned place preference
OT  - lentiviral vector
OT  - miR124a
OT  - microRNA
OT  - siRNA
EDAT- 2013/04/23 06:00
MHDA- 2014/01/24 06:00
CRDT- 2013/04/23 06:00
PHST- 2012/11/12 00:00 [received]
PHST- 2013/03/21 00:00 [revised]
PHST- 2013/03/22 00:00 [accepted]
PHST- 2013/04/23 06:00 [entrez]
PHST- 2013/04/23 06:00 [pubmed]
PHST- 2014/01/24 06:00 [medline]
AID - 10.1111/ejn.12228 [doi]
PST - ppublish
SO  - Eur J Neurosci. 2013 Jul;38(2):2328-37. doi: 10.1111/ejn.12228. Epub 2013 Apr 19.