PMID- 23602639
OWN - NLM
STAT- MEDLINE
DCOM- 20140121
LR  - 20190107
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1300
DP  - 2013 Jul 26
TI  - Chromatographic determination of aliphatic aldehydes in human serum after 
      pre-column derivatization using 2,2'-furil, a novel fluorogenic reagent.
PG  - 199-203
LID - S0021-9673(13)00498-6 [pii]
LID - 10.1016/j.chroma.2013.03.033 [doi]
AB  - A novel, highly sensitive and selective fluorimetric liquid chromatographic 
      method for simultaneous determination of medium chain aliphatic aldehydes was 
      developed. The method was based on the derivatization of aliphatic aldehydes with 
      1,2-di(2-furyl)-1,2-ethanedione (2,2'-furil), a novel fluorogenic reagent, to 
      form highly fluorescent difurylimidazole derivatives. The fluorescence 
      derivatives were separated in less than 20min on a reversed-phase ODS column 
      using an isocratic elution with a mixture of methanol-water (80:20, v/v%). The 
      detection limits were from 0.19 to 0.50nM (1-10fmol/injection) at a 
      signal-to-noise ratio (S/N) of 3. This method was successfully applied for 
      monitoring of aliphatic aldehydes in healthy human sera by a simple pretreatment 
      procedure without interferences from serum constituents.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Fathy Bakr Ali, Marwa
AU  - Fathy Bakr Ali M
AD  - Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut 
      University, Assiut 71526, Egypt.
FAU - Kishikawa, Naoya
AU  - Kishikawa N
FAU - Ohyama, Kaname
AU  - Ohyama K
FAU - Abdel-Mageed Mohamed, Horria
AU  - Abdel-Mageed Mohamed H
FAU - Mohamed Abdel-Wadood, Hanaa
AU  - Mohamed Abdel-Wadood H
FAU - Mohamed Mohamed, Ashraf
AU  - Mohamed Mohamed A
FAU - Kuroda, Naotaka
AU  - Kuroda N
LA  - eng
PT  - Journal Article
DEP - 20130326
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Aldehydes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Furans)
RN  - 3AFE4C3P1F (alpha-furil)
SB  - IM
MH  - Aldehydes/*blood/chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Fluorescent Dyes/*chemistry
MH  - Furans/*chemistry
MH  - Humans
MH  - Limit of Detection
MH  - Linear Models
MH  - Reproducibility of Results
MH  - Signal-To-Noise Ratio
OTO - NOTNLM
OT  - 1,2-Di(2-furyl)-1,2-ethanedione (2,2'-furil)
OT  - Aliphatic aldehydes (lipid peroxidation)
OT  - Difurylimidazole derivatives
OT  - Pre-column fluorogenic derivatization
OT  - Serum analysis
EDAT- 2013/04/23 06:00
MHDA- 2014/01/22 06:00
CRDT- 2013/04/23 06:00
PHST- 2013/01/25 00:00 [received]
PHST- 2013/03/08 00:00 [revised]
PHST- 2013/03/11 00:00 [accepted]
PHST- 2013/04/23 06:00 [entrez]
PHST- 2013/04/23 06:00 [pubmed]
PHST- 2014/01/22 06:00 [medline]
AID - S0021-9673(13)00498-6 [pii]
AID - 10.1016/j.chroma.2013.03.033 [doi]
PST - ppublish
SO  - J Chromatogr A. 2013 Jul 26;1300:199-203. doi: 10.1016/j.chroma.2013.03.033. Epub 
      2013 Mar 26.