PMID- 23602886
OWN - NLM
STAT- MEDLINE
DCOM- 20140219
LR  - 20220311
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 245
DP  - 2013 Aug 15
TI  - Reduced GABAA receptor alpha6 expression in the trigeminal ganglion enhanced 
      myofascial nociceptive response.
PG  - 1-11
LID - S0306-4522(13)00321-7 [pii]
LID - 10.1016/j.neuroscience.2013.04.003 [doi]
AB  - Activation of the GABAA receptor results in inhibition of neuronal activity. One 
      subunit of this multi-subunit receptor termed alpha 6 (Gabralpha6) contributed to 
      inflammatory temporomandibular joint (TMJ) nociception but TMJ disorders often 
      include myofascial pain. To address Gabralpha6 role in myofascial pain we 
      hypothesized that Gabralpha6 has an inhibitory role in myofascial nociceptive 
      responses similar to inflammatory TMJ arthritis. To test this hypothesis a, 
      myofascial nociceptive response was induced by placing a ligature bilaterally on 
      the tendon attachment of the anterior superficial part of a male rat's masseter 
      muscle. Four days after ligature placement Gabralpha6 expression was reduced by 
      infusing the trigeminal ganglia (TG) with small interfering RNA (siRNA) having 
      homology to either the Gabralpha6 gene (Gabralpha6 siRNA) or no known gene (control 
      siRNA). After siRNA infusion nociceptive behavioral responses were measured, 
      i.e., feeding behavior and head withdrawal after pressing upon the region above 
      the ligature with von Frey filaments. Neuronal activity in the TG and trigeminal 
      nucleus caudalis and upper cervical region (Vc-C1) was measured by quantitating 
      the amount of phosphorylated extracellular signal-regulated kinase (p-ERK). Total 
      Gabralpha6 and GABAA receptor contents in the TG and Vc-C1 were determined. Gabralpha6 
      siRNA infusion reduced Gabralpha6 and GABAA receptor expression and significantly 
      increased the nociceptive response in both nociceptive assays. Gabralpha6 siRNA 
      infusion also significantly increased TG p-ERK expression of the ligated rats. 
      From these results we conclude GABAA receptors consisting of the Gabralpha6 subunit 
      inhibit TG nociceptive sensory afferents in the trigeminal pathway and have an 
      important role in the regulation of myofascial nociception.
CI  - Copyright (c) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Kramer, P R
AU  - Kramer PR
AD  - Department of Biomedical Sciences, Texas A&M Health Science Center Baylor College 
      of Dentistry, 3302 Gaston Avenue, Dallas, TX 75246, United States. 
      pkramer@bcd.tamhsc.edu
FAU - Bellinger, L L
AU  - Bellinger LL
LA  - eng
GR  - R01 DE016059/DE/NIDCR NIH HHS/United States
GR  - R01 DE022129/DE/NIDCR NIH HHS/United States
GR  - DE022129/DE/NIDCR NIH HHS/United States
GR  - DE016059/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130418
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Gabra6 protein, rat)
RN  - 0 (Protein Subunits)
RN  - 0 (Receptors, GABA-A)
SB  - IM
MH  - Animals
MH  - Facial Pain/*metabolism/pathology
MH  - *Gene Expression Regulation
MH  - Male
MH  - Nociception/*physiology
MH  - Pain Measurement/methods
MH  - Protein Subunits/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, GABA-A/*biosynthesis/genetics
MH  - Trigeminal Ganglion/*metabolism
PMC - PMC3740526
MID - NIHMS470337
EDAT- 2013/04/23 06:00
MHDA- 2014/02/20 06:00
PMCR- 2014/08/15
CRDT- 2013/04/23 06:00
PHST- 2012/12/11 00:00 [received]
PHST- 2013/04/01 00:00 [revised]
PHST- 2013/04/02 00:00 [accepted]
PHST- 2013/04/23 06:00 [entrez]
PHST- 2013/04/23 06:00 [pubmed]
PHST- 2014/02/20 06:00 [medline]
PHST- 2014/08/15 00:00 [pmc-release]
AID - S0306-4522(13)00321-7 [pii]
AID - 10.1016/j.neuroscience.2013.04.003 [doi]
PST - ppublish
SO  - Neuroscience. 2013 Aug 15;245:1-11. doi: 10.1016/j.neuroscience.2013.04.003. Epub 
      2013 Apr 18.