PMID- 23607629
OWN - NLM
STAT- MEDLINE
DCOM- 20130805
LR  - 20220317
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 173
IP  - 1
DP  - 2013 Jul
TI  - Aberrant expression of microRNAs in T cells from patients with ankylosing 
      spondylitis contributes to the immunopathogenesis.
PG  - 47-57
LID - 10.1111/cei.12089 [doi]
AB  - Ankylosing spondylitis (AS) is a chronic inflammatory disorder characterized by 
      dysregulated T cells. We hypothesized that the aberrant expression of microRNAs 
      (miRNAs) in AS T cells involved in the pathogenesis of AS. The expression profile 
      of 270 miRNAs in T cells from five AS patients and five healthy controls were 
      analysed by real-time polymerase chain reaction (PCR). Thirteen miRNAs were found 
      potentially differential expression. After validation, we confirmed that miR-16, 
      miR-221 and let-7i were over-expressed in AS T cells and the expression of 
      miR-221 and let-7i were correlated positively with the Bath Ankylosing 
      Spondylitis Radiology Index (BASRI) of lumbar spine in AS patients. The protein 
      molecules regulated by miR-16, miR-221 and let-7i were measured by Western 
      blotting. We found that the protein levels of Toll-like receptor-4 (TLR-4), a 
      target of let-7i, in T cells from AS patients were decreased. In addition, the 
      mRNA expression of interferon (IFN)-gamma was elevated in AS T cells. 
      Lipopolysaccharide (LPS), a TLR-4 agonist, inhibited IFN-gamma secretion by 
      anti-CD3(+) anti-CD28 antibodies-stimulated normal T cells but not AS T cells. In 
      the transfection studies, we found the increased expression of let-7i enhanced 
      IFN-gamma production by anti-CD3(+) anti-CD28(+) lipopolysaccharide (LPS)-stimulated 
      normal T cells. In contrast, the decreased expression of let-7i suppressed IFN-gamma 
      production by anti-CD3(+) anti-CD28(+) LPS-stimulated AS T cells. In conclusion, 
      we found that miR-16, miR-221 and let-7i were over-expressed in AS T cells, but 
      only miR-221 and let-7i were associated with BASRI of lumbar spine. In the 
      functional studies, the increased let-7i expression facilitated the T helper type 
      1 (IFN-gamma) immune response in T cells.
CI  - (c) 2013 British Society for Immunology.
FAU - Lai, N-S
AU  - Lai NS
AD  - Division of Allergy, Immunology and Rheumatology, Buddhist Dalin Tzu Chi General 
      Hospital, Taiwan.
FAU - Yu, H-C
AU  - Yu HC
FAU - Chen, H-C
AU  - Chen HC
FAU - Yu, C-L
AU  - Yu CL
FAU - Huang, H-B
AU  - Huang HB
FAU - Lu, M-C
AU  - Lu MC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MIRN16 microRNA, human)
RN  - 0 (MIRN221 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (mirnlet7 microRNA, human)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Arthritis, Rheumatoid/metabolism
MH  - Case-Control Studies
MH  - Cells, Cultured/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interferon-gamma/biosynthesis/genetics/metabolism
MH  - Jurkat Cells/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lumbar Vertebrae/diagnostic imaging
MH  - Lupus Erythematosus, Systemic/metabolism
MH  - Male
MH  - MicroRNAs/antagonists & inhibitors/*biosynthesis/genetics/physiology
MH  - Middle Aged
MH  - RNA, Messenger/biosynthesis
MH  - Radiography
MH  - Severity of Illness Index
MH  - Spondylitis, Ankylosing/diagnostic imaging/etiology/genetics/*immunology
MH  - Th1 Cells/immunology/*metabolism
MH  - Toll-Like Receptor 4/biosynthesis/genetics
MH  - Young Adult
PMC - PMC3694534
EDAT- 2013/04/24 06:00
MHDA- 2013/08/06 06:00
PMCR- 2014/07/01
CRDT- 2013/04/24 06:00
PHST- 2013/02/04 00:00 [accepted]
PHST- 2013/04/24 06:00 [entrez]
PHST- 2013/04/24 06:00 [pubmed]
PHST- 2013/08/06 06:00 [medline]
PHST- 2014/07/01 00:00 [pmc-release]
AID - 10.1111/cei.12089 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2013 Jul;173(1):47-57. doi: 10.1111/cei.12089.