PMID- 23608488
OWN - NLM
STAT- MEDLINE
DCOM- 20130719
LR  - 20211021
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 319
IP  - 10
DP  - 2013 Jun 10
TI  - IL-4 inhibition of IL-1 induced Matrix metalloproteinase-3 (MMP-3) expression in 
      human fibroblasts involves decreased AP-1 activation via negative crosstalk 
      involving of Jun N-terminal kinase (JNK).
PG  - 1398-408
LID - S0014-4827(13)00169-9 [pii]
LID - 10.1016/j.yexcr.2013.04.010 [doi]
AB  - Matrix metalloproteinase-3 (MMP-3) over-expression is associated with tissue 
      destruction in the context of chronic inflammation. Previous studies showed that 
      IL-4 inhibits induction of MMP-3 by IL-1beta, and suggested that AP-1 might be 
      involved. Here we show that IL-1 induced binding of transcription factor AP-1 to 
      the MMP-3 promoter consists primarily of c-Jun, JunB, and c-Fos and that binding 
      of c-Jun and c-Fos is inhibited by the combination of cytokines while binding of 
      Jun B is not. Mutation of the AP-1 site in the MMP-3 promoter decreased the 
      ability of IL-4 to inhibit its transcription in transfected MG-63 cells. Western 
      blotting showed that both cytokines activate Jun N-terminal kinase (JNK), but 
      with somewhat different kinetics, and that activation of JNK by both cytokines 
      individually is inhibited by the combination. These results indicate that IL-4 
      inhibition of MMP-3 expression is associated with reduction of IL-1 induced 
      binding of active forms of the AP-1 dimer, while less active JunB-containing 
      dimers remain, and suggest that these changes are associated with decreased 
      activation of JNK.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Chambers, Mariah
AU  - Chambers M
AD  - Department of Biochemistry and Molecular Biology, Philadelphia College of 
      Osteopathic Medicine, 4170 City Avenue, Philadelphia, PA 19131, USA.
FAU - Kirkpatrick, Garrett
AU  - Kirkpatrick G
FAU - Evans, Michel
AU  - Evans M
FAU - Gorski, Grzegorz
AU  - Gorski G
FAU - Foster, Sara
AU  - Foster S
FAU - Borghaei, Ruth C
AU  - Borghaei RC
LA  - eng
GR  - R15 DE016277/DE/NIDCR NIH HHS/United States
GR  - R15DE16277/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130419
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (FOSB protein, human)
RN  - 0 (Interleukin-1)
RN  - 0 (JunB protein, human)
RN  - 0 (JunD protein, human)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transcription Factors)
RN  - 207137-56-2 (Interleukin-4)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 8)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.7 (MMP1 protein, human)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Binding Sites
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Enzyme Activation
MH  - Fibroblasts/drug effects/enzymology/metabolism
MH  - Foreskin/cytology
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Interleukin-4/*pharmacology
MH  - Male
MH  - Matrix Metalloproteinase 1/genetics/metabolism
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Mitogen-Activated Protein Kinase 8/genetics/*metabolism
MH  - Periodontitis/enzymology/metabolism/pathology
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Protein Multimerization
MH  - Proto-Oncogene Proteins c-fos/genetics/metabolism
MH  - Proto-Oncogene Proteins c-jun/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Transcription Factor AP-1/*metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Transcriptional Activation
MH  - Transfection
PMC - PMC3757497
MID - NIHMS470770
EDAT- 2013/04/24 06:00
MHDA- 2013/07/20 06:00
PMCR- 2014/06/10
CRDT- 2013/04/24 06:00
PHST- 2012/07/31 00:00 [received]
PHST- 2013/04/01 00:00 [revised]
PHST- 2013/04/05 00:00 [accepted]
PHST- 2013/04/24 06:00 [entrez]
PHST- 2013/04/24 06:00 [pubmed]
PHST- 2013/07/20 06:00 [medline]
PHST- 2014/06/10 00:00 [pmc-release]
AID - S0014-4827(13)00169-9 [pii]
AID - 10.1016/j.yexcr.2013.04.010 [doi]
PST - ppublish
SO  - Exp Cell Res. 2013 Jun 10;319(10):1398-408. doi: 10.1016/j.yexcr.2013.04.010. 
      Epub 2013 Apr 19.