PMID- 23609495
OWN - NLM
STAT- MEDLINE
DCOM- 20150916
LR  - 20240318
IS  - 1422-0067 (Print)
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 14
IP  - 4
DP  - 2013 Apr 22
TI  - Single nucleotide polymorphisms in the leptin-a gene and associations with growth 
      traits in the orange-spotted grouper (Epinephelus coioides).
PG  - 8625-37
LID - 10.3390/ijms14048625 [doi]
AB  - Leptin is a multifunctional protein involved in processes such as body weight 
      regulation, energy expenditure, fat metabolism, food intake, and appetite 
      regulation. Duplicate leptin genes, leptin-a and leptin-b, were previously 
      detected in the orange-spotted grouper. In this study, we cloned the full-length 
      open reading frame (ORF) of the leptin-a gene in the orange-spotted grouper, 
      searched for polymorphisms, and performed association analyses between these 
      polymorphisms and seven growth traits. Six polymorphisms, consisting of 2 SNPs in 
      intron 1 (c.182T > G, c.183G > T) and 4 SNPs in exon 2 (c.339C > G, c.345C > T, 
      c.447G > A, c.531C > T), were identified and genotyped in 200 individuals. The 
      c.182T > G and c.183G > T polymorphisms showed complete linkage and were analyzed 
      together. Association analyses revealed that the c.182 + 183TG > GT polymorphism 
      was significantly associated with body weight (BWT) and body width (BWH), with 
      the AB (TG/GT) genotype showing positive effects on growth traits. Additionally, 
      the SNP c.447G > A was significantly associated with BWT, BWH, overall length 
      (OL), trunk width (TW), and head length (HL), with the GA genotype displaying 
      positive effects on growth traits. The c.531C > T SNP showed a close association 
      between the TT genotype and decreased growth. Our results demonstrate that 
      several polymorphisms in the leptin-a gene are associated with growth traits and 
      can be used for marker-assisted selection (MAS) in orange-spotted grouper 
      populations.
FAU - Wei, Yun
AU  - Wei Y
AD  - Key Laboratory of Tropical Biology Resources, Ministry of Education, College of 
      Ocean, Hainan University, Haikou 570228, China. huanghai74@126.com.
FAU - Huang, Hai
AU  - Huang H
FAU - Meng, Zining
AU  - Meng Z
FAU - Zhang, Yong
AU  - Zhang Y
FAU - Luo, Jian
AU  - Luo J
FAU - Chen, Guohua
AU  - Chen G
FAU - Lin, Haoran
AU  - Lin H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130422
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Fish Proteins)
RN  - 0 (Genetic Markers)
RN  - 0 (Leptin)
SB  - IM
MH  - Animals
MH  - Bass/*genetics/*growth & development
MH  - Body Weight/genetics
MH  - Female
MH  - Fish Proteins/*genetics
MH  - Genetic Association Studies
MH  - Genetic Markers
MH  - Leptin/*genetics
MH  - Male
MH  - *Polymorphism, Single Nucleotide
PMC - PMC3645766
EDAT- 2013/04/24 06:00
MHDA- 2013/04/24 06:01
PMCR- 2013/04/01
CRDT- 2013/04/24 06:00
PHST- 2013/02/07 00:00 [received]
PHST- 2013/04/11 00:00 [revised]
PHST- 2013/04/16 00:00 [accepted]
PHST- 2013/04/24 06:00 [entrez]
PHST- 2013/04/24 06:00 [pubmed]
PHST- 2013/04/24 06:01 [medline]
PHST- 2013/04/01 00:00 [pmc-release]
AID - ijms14048625 [pii]
AID - ijms-14-08625 [pii]
AID - 10.3390/ijms14048625 [doi]
PST - epublish
SO  - Int J Mol Sci. 2013 Apr 22;14(4):8625-37. doi: 10.3390/ijms14048625.