PMID- 23611163
OWN - NLM
STAT- MEDLINE
DCOM- 20140921
LR  - 20130605
IS  - 1029-2470 (Electronic)
IS  - 1029-2470 (Linking)
VI  - 47
IP  - 6-7
DP  - 2013 Jul
TI  - Long-term stability of parameters of antioxidant status in human serum.
PG  - 535-40
LID - 10.3109/10715762.2013.797969 [doi]
AB  - The antioxidant status of serum or plasma can be determined using several 
      commercially available assays. Here, four different assays, total antioxidant 
      status (TAS), its second-generation assay (TAS2), biological antioxidant 
      potential (BAP), and enzymatic assay using horseradish peroxidase (EAOC), were 
      applied on human serum samples to test the temperature stability of antioxidants, 
      upon storage of serum for 12 months. The two or three most commonly used 
      temperatures for storage, that is, - 20, - 70 (or - 80), and - 196 degrees C, were 
      selected. The general conclusion is that all assays were stable at the 
      temperatures tested. In addition, there were almost no statistically significant 
      differences between the samples stored at different temperatures. Only the rank 
      order of the EAOC assay was not very good in samples stored at - 20 degrees C. Also three 
      components contributing to the total antioxidant capacity, uric acid, creatinine 
      and bilirubin, showed no statistically significant differences between the 
      temperatures. Therefore, storage at - 20 degrees C is sufficient to maintain a proper 
      assay outcome of most of the total antioxidant assays, although storage at - 
      70/80 degrees C is to be preferred for longer storage times.
FAU - Jansen, E H J M
AU  - Jansen EH
AD  - Centre for Health Protection, National Institute of Public Health and the 
      Environment, Bilthoven, The Netherlands. eugene.jansen@rivm.nl
FAU - Beekhof, P K
AU  - Beekhof PK
FAU - Cremers, J W J M
AU  - Cremers JW
FAU - Viezeliene, D
AU  - Viezeliene D
FAU - Muzakova, V
AU  - Muzakova V
FAU - Skalicky, J
AU  - Skalicky J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130515
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (Antioxidants)
RN  - 268B43MJ25 (Uric Acid)
RN  - AYI8EX34EU (Creatinine)
RN  - RFM9X3LJ49 (Bilirubin)
SB  - IM
MH  - Antioxidants/chemistry/*metabolism
MH  - Bilirubin/chemistry/*metabolism
MH  - Creatinine/chemistry/*metabolism
MH  - Humans
MH  - Serum/*chemistry/metabolism
MH  - Temperature
MH  - Time Factors
MH  - Uric Acid/chemistry/*metabolism
EDAT- 2013/04/25 06:00
MHDA- 2014/09/23 06:00
CRDT- 2013/04/25 06:00
PHST- 2013/04/25 06:00 [entrez]
PHST- 2013/04/25 06:00 [pubmed]
PHST- 2014/09/23 06:00 [medline]
AID - 10.3109/10715762.2013.797969 [doi]
PST - ppublish
SO  - Free Radic Res. 2013 Jul;47(6-7):535-40. doi: 10.3109/10715762.2013.797969. Epub 
      2013 May 15.