PMID- 23613272
OWN - NLM
STAT- MEDLINE
DCOM- 20140128
LR  - 20211021
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 162
IP  - 2
DP  - 2013 Jun
TI  - The endoplasmic reticulum is a reservoir for WAVE/SCAR regulatory complex 
      signaling in the Arabidopsis leaf.
PG  - 689-706
LID - 10.1104/pp.113.217422 [doi]
AB  - During plant cell morphogenesis, signal transduction and cytoskeletal dynamics 
      interact to locally organize the cytoplasm and define the geometry of cell 
      expansion. The WAVE/SCAR (for WASP family verprolin homologous/suppressor of 
      cyclic AMP receptor) regulatory complex (W/SRC) is an evolutionarily conserved 
      heteromeric protein complex. Within the plant kingdom W/SRC is a broadly used 
      effector that converts Rho-of-Plants (ROP)/Rac small GTPase signals into 
      Actin-Related Protein2/3 and actin-dependent growth responses. Although the 
      components and biochemistry of the W/SRC pathway are well understood, a basic 
      understanding of how cells partition W/SRC into active and inactive pools is 
      lacking. In this paper, we report that the endoplasmic reticulum (ER) is an 
      important organelle for W/SRC regulation. We determined that a large 
      intracellular pool of the core W/SRC subunit NAP1, like the known positive 
      regulator of W/SRC, the DOCK family guanine nucleotide-exchange factor SPIKE1 
      (SPK1), localizes to the surface of the ER. The ER-associated NAP1 is inactive 
      because it displays little colocalization with the actin network, and ER 
      localization requires neither activating signals from SPK1 nor a physical 
      association with its W/SRC-binding partner, SRA1. Our results indicate that in 
      Arabidopsis (Arabidopsis thaliana) leaf pavement cells and trichomes, the ER is a 
      reservoir for W/SRC signaling and may have a key role in the early steps of W/SRC 
      assembly and/or activation.
FAU - Zhang, Chunhua
AU  - Zhang C
AD  - Department of Agronomy and Department of Biological Sciences, Purdue University, 
      West Lafayette, Indiana 47907, USA.
FAU - Mallery, Eileen
AU  - Mallery E
FAU - Reagan, Sara
AU  - Reagan S
FAU - Boyko, Vitaly P
AU  - Boyko VP
FAU - Kotchoni, Simeon O
AU  - Kotchoni SO
FAU - Szymanski, Daniel B
AU  - Szymanski DB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130423
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Actins)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (DIS3 protein, Arabidopsis)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (SPIKE1 protein, Arabidopsis)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.3 (NAP1 protein, Arabidopsis)
SB  - IM
MH  - Actins/metabolism
MH  - Adenosine Triphosphatases/genetics/*metabolism
MH  - Arabidopsis/genetics/*metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Endoplasmic Reticulum/genetics/*metabolism
MH  - Intracellular Membranes/metabolism
MH  - Multiprotein Complexes/metabolism
MH  - Plant Leaves/genetics/*metabolism
MH  - Plants, Genetically Modified
MH  - Signal Transduction
MH  - Trichomes/metabolism
PMC - PMC3668063
EDAT- 2013/04/25 06:00
MHDA- 2014/01/29 06:00
PMCR- 2013/04/23
CRDT- 2013/04/25 06:00
PHST- 2013/04/25 06:00 [entrez]
PHST- 2013/04/25 06:00 [pubmed]
PHST- 2014/01/29 06:00 [medline]
PHST- 2013/04/23 00:00 [pmc-release]
AID - pp.113.217422 [pii]
AID - 217422 [pii]
AID - 10.1104/pp.113.217422 [doi]
PST - ppublish
SO  - Plant Physiol. 2013 Jun;162(2):689-706. doi: 10.1104/pp.113.217422. Epub 2013 Apr 
      23.