PMID- 23613363
OWN - NLM
STAT- MEDLINE
DCOM- 20130618
LR  - 20220321
IS  - 1529-0131 (Electronic)
IS  - 0004-3591 (Linking)
VI  - 65
IP  - 5
DP  - 2013 May
TI  - Adipose-derived mesenchymal stem cells exert antiinflammatory effects on 
      chondrocytes and synoviocytes from osteoarthritis patients through prostaglandin 
      E2.
PG  - 1271-81
LID - 10.1002/art.37908 [doi]
AB  - OBJECTIVE: To examine the effect of different sources of good manufacturing 
      practice clinical grade adipose-derived mesenchymal stem cells (AD-MSCs) on 
      inflammatory factors in osteoarthritic (OA) chondrocytes and synoviocytes. 
      METHODS: AD-MSCs from infrapatellar Hoffa fat, subcutaneous (SC) hip fat, and SC 
      abdominal fat were cocultured in Transwells with chondrocytes or synoviocytes. 
      Inflammatory factors (interleukin-1beta [IL-1beta], tumor necrosis factor alpha, IL-6, 
      CXCL1/growth-related oncogene alpha, CXCL8/IL-8, CCL2/monocyte chemotactic protein 1, 
      CCL3/macrophage inflammatory protein 1alpha, and CCL5/RANTES) were evaluated by 
      quantitative reverse transcription-polymerase chain reaction or multiplex 
      bead-based immunoassay. The role of different immunomodulators was analyzed. 
      RESULTS: All the inflammatory factors analyzed were down-modulated at the 
      messenger RNA or protein level independently by all 3 AD-MSC sources or by 
      allogeneic AD-MSCs used in coculture with chondrocytes or synoviocytes. 
      Inflammatory factor down-modulation was observed only when AD-MSCs were 
      cocultured with chondrocytes or synoviocytes that produced high levels of 
      inflammatory factors, but no effect was observed in cells that produced low 
      levels of those factors, thus highlighting a dependence of the AD-MSC effect on 
      existing inflammation. The immunomodulators IL-10, IL-1 receptor antagonist, 
      fibroblast growth factor 2, indoleamine 2,3-dioxygenase 1, and galectin 1 were 
      not involved in AD-MSC effects, whereas the cyclooxygenase 2 
      (COX-2)/prostaglandin E2 (PGE2 ) pathway exerted a role in the mechanism of 
      antiinflammatory AD-MSC action. CONCLUSION: The antiinflammatory effects of 
      AD-MSCs are probably not dependent on AD-MSC adipose tissue sources and donors 
      but rather on the inflammatory status of OA chondrocytes and synoviocytes. 
      AD-MSCs seem to be able to sense and respond to the local environment. Even 
      though a combination of different molecules may be involved in AD-MSC effects, 
      the COX-2/PGE2 pathway may play a role, suggesting that AD-MSCs may be useful for 
      therapies in osteoarticular diseases.
CI  - Copyright (c) 2013 by the American College of Rheumatology.
FAU - Manferdini, Cristina
AU  - Manferdini C
AD  - Istituto Ortopedico Rizzoli, Bologna, Italy.
FAU - Maumus, Marie
AU  - Maumus M
FAU - Gabusi, Elena
AU  - Gabusi E
FAU - Piacentini, Anna
AU  - Piacentini A
FAU - Filardo, Giuseppe
AU  - Filardo G
FAU - Peyrafitte, Julie-Anne
AU  - Peyrafitte JA
FAU - Jorgensen, Christian
AU  - Jorgensen C
FAU - Bourin, Philippe
AU  - Bourin P
FAU - Fleury-Cappellesso, Sandrine
AU  - Fleury-Cappellesso S
FAU - Facchini, Andrea
AU  - Facchini A
FAU - Noel, Daniele
AU  - Noel D
FAU - Lisignoli, Gina
AU  - Lisignoli G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Biomarkers)
RN  - 0 (Chemokines)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Adipocytes/*cytology
MH  - Aged
MH  - Biomarkers/metabolism
MH  - Cartilage, Articular/pathology
MH  - Cells, Cultured
MH  - Chemokines/genetics/metabolism
MH  - Chondrocytes/*cytology/metabolism
MH  - Coculture Techniques
MH  - Dinoprostone/*metabolism
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Osteoarthritis/*pathology
MH  - Synovial Membrane/*cytology/metabolism
EDAT- 2013/04/25 06:00
MHDA- 2013/06/19 06:00
CRDT- 2013/04/25 06:00
PHST- 2012/09/17 00:00 [received]
PHST- 2013/02/12 00:00 [accepted]
PHST- 2013/04/25 06:00 [entrez]
PHST- 2013/04/25 06:00 [pubmed]
PHST- 2013/06/19 06:00 [medline]
AID - 10.1002/art.37908 [doi]
PST - ppublish
SO  - Arthritis Rheum. 2013 May;65(5):1271-81. doi: 10.1002/art.37908.