PMID- 23613483
OWN - NLM
STAT- MEDLINE
DCOM- 20131111
LR  - 20171116
IS  - 1477-0903 (Electronic)
IS  - 0960-3271 (Linking)
VI  - 32
IP  - 4
DP  - 2013 Apr
TI  - Effect of diindolylmethane on Ca(2+) homeostasis and viability in MDCK renal 
      tubular cells.
PG  - 344-53
LID - 10.1177/0960327112462727 [doi]
AB  - The effect of the natural product diindolylmethane (DIM) on cytosolic Ca(2+) 
      concentrations ([Ca(2+)]i) and viability in MDCK renal tubular cells was 
      explored. The Ca(2+)-sensitive fluorescent dye fura-2 was applied to measure 
      [Ca(2+)]i. DIM at concentrations 1-50 muM induced a [Ca(2+)]i rise in a 
      concentration-dependent manner. The response was reduced partly by removing 
      Ca(2+). DIM induced Mn(2+) influx leading to quenching of fura-2 fluorescence. 
      DIM-evoked Ca(2+) entry was suppressed by nifedipine, econazole, SK&F96365 and 
      protein kinase C modulators. In the absence of extracellular Ca(2+), incubation 
      with the endoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (TG) or 
      2,5-di-tert-butylhydroquinone (BHQ) greatly inhibited DIM-induced [Ca(2+)]i rise. 
      Incubation with DIM abolished TG or BHQ-induced [Ca(2+)]i rise. Inhibition of 
      phospholipase C with U73122 reduced DIM-induced [Ca(2+)]i rise by 50%. At 1, 10, 
      40 and 50 muM, DIM slightly enhanced cell proliferation. The effect of 50 muM DIM 
      was reversed by chelating cytosolic Ca(2+) with 
      1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid. In sum, in MDCK cells, 
      DIM induced a [Ca(2+)]i rise by evoking phospholipase C-dependent Ca(2+) release 
      from the endoplasmic reticulum and Ca(2+) entry via protein kinase C-sensitive 
      store-operated Ca(2+) channels. DIM did not induce cell death.
FAU - Fang, Y-C
AU  - Fang YC
AD  - Department of Laboratory Medicine, Zuoying Armed Forces General Hospital, 
      Kaohsiung, Taiwan.
FAU - Chou, C-T
AU  - Chou CT
FAU - Chi, C-C
AU  - Chi CC
FAU - Lin, K-L
AU  - Lin KL
FAU - Li, Y-D
AU  - Li YD
FAU - Cheng, H-H
AU  - Cheng HH
FAU - Lu, Y-C
AU  - Lu YC
FAU - Cheng, J-S
AU  - Cheng JS
FAU - Kuo, C-C
AU  - Kuo CC
FAU - Jan, C-R
AU  - Jan CR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (Biological Products)
RN  - 0 (Indoles)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - SSZ9HQT61Z (3,3'-diindolylmethane)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Biological Products/*pharmacology
MH  - Calcium/*metabolism
MH  - Cell Survival/drug effects
MH  - Dogs
MH  - Endoplasmic Reticulum/drug effects/metabolism
MH  - Homeostasis/drug effects
MH  - Indoles/*pharmacology
MH  - Kidney Tubules/cytology
MH  - Madin Darby Canine Kidney Cells
MH  - Type C Phospholipases/metabolism
EDAT- 2013/04/25 06:00
MHDA- 2013/11/12 06:00
CRDT- 2013/04/25 06:00
PHST- 2013/04/25 06:00 [entrez]
PHST- 2013/04/25 06:00 [pubmed]
PHST- 2013/11/12 06:00 [medline]
AID - 0960327112462727 [pii]
AID - 10.1177/0960327112462727 [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2013 Apr;32(4):344-53. doi: 10.1177/0960327112462727.