PMID- 23613991
OWN - NLM
STAT- MEDLINE
DCOM- 20131111
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 4
DP  - 2013
TI  - Genome-wide analysis of alternative splicing during dendritic cell response to a 
      bacterial challenge.
PG  - e61975
LID - 10.1371/journal.pone.0061975 [doi]
LID - e61975
AB  - The immune system relies on the plasticity of its components to produce 
      appropriate responses to frequent environmental challenges. Dendritic cells (DCs) 
      are critical initiators of innate immunity and orchestrate the later and more 
      specific adaptive immunity. The generation of diversity in transcriptional 
      programs is central for effective immune responses. Alternative splicing is 
      widely considered a key generator of transcriptional and proteomic complexity, 
      but its role has been rarely addressed systematically in immune cells. Here we 
      used splicing-sensitive arrays to assess genome-wide gene- and exon-level 
      expression profiles in human DCs in response to a bacterial challenge. We find 
      widespread alternative splicing events and splicing factor transcriptional 
      signatures induced by an E. coli challenge to human DCs. Alternative splicing 
      acts in concert with transcriptional modulation, but these two mechanisms of gene 
      regulation affect primarily distinct functional gene groups. Alternative splicing 
      is likely to have an important role in DC immunobiology because it affects genes 
      known to be involved in DC development, endocytosis, antigen presentation and 
      cell cycle arrest.
FAU - Rodrigues, Raquel
AU  - Rodrigues R
AD  - Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 
      Lisboa, Portugal.
FAU - Grosso, Ana Rita
AU  - Grosso AR
FAU - Moita, Luis
AU  - Moita L
LA  - eng
SI  - GEO/GSE42561
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130417
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Alternative Splicing/*genetics
MH  - Dendritic Cells/*immunology/*microbiology
MH  - Escherichia coli/*immunology
MH  - Gene Expression Profiling
MH  - Genome, Human/*genetics
MH  - Humans
MH  - Oligonucleotide Array Sequence Analysis
MH  - Protein Isoforms/genetics/metabolism
MH  - RNA-Binding Proteins/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Reproducibility of Results
PMC - PMC3629138
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/04/25 06:00
MHDA- 2013/11/12 06:00
PMCR- 2013/04/17
CRDT- 2013/04/25 06:00
PHST- 2012/08/23 00:00 [received]
PHST- 2013/03/13 00:00 [accepted]
PHST- 2013/04/25 06:00 [entrez]
PHST- 2013/04/25 06:00 [pubmed]
PHST- 2013/11/12 06:00 [medline]
PHST- 2013/04/17 00:00 [pmc-release]
AID - PONE-D-12-25301 [pii]
AID - 10.1371/journal.pone.0061975 [doi]
PST - epublish
SO  - PLoS One. 2013 Apr 17;8(4):e61975. doi: 10.1371/journal.pone.0061975. Print 2013.