PMID- 23614687
OWN - NLM
STAT- MEDLINE
DCOM- 20131122
LR  - 20181202
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 85
IP  - 10
DP  - 2013 May 21
TI  - Fluorescent Ru(phen)3(2+)-doped silica nanoparticles-based ICTS sensor for 
      quantitative detection of enrofloxacin residues in chicken meat.
PG  - 5120-8
LID - 10.1021/ac400502v [doi]
AB  - A Ru(phen)3(2+)-doped silica fluorescent nanoparticle (FN)-based 
      immunochromatographic test strip (ICTS) sensor was developed for rapid, high 
      sensitivity, easy to use, and low cost quantitative detection of enrofloxacin 
      (ENR) residues in chicken meat. The fluorescence signal intensity of the FNs at 
      the test line (FI(T)) and control line (FI(C)) was determined with a prototype of 
      a portable fluorescent strip reader. Unique properties of Ru(phen)3(2+) doped 
      silica nanoparticles (e.g., large Stokes shift, high emission quantum yield, and 
      long fluorescence lifetime) were combined with the advantages of ICTS and an easy 
      to make portable fluorescent strip reader. The signal was based on FI(T)/FI(C) 
      ratio to effectively eliminate strip to strip variation and matrix effects. 
      Various parameters that influenced the strip were investigated and optimized. 
      Quantitative ENR detection with the FNs ICTS sensor using 80 muL sample took only 
      20 min, which is faster than the commercial ELISA kit (that took 90 min). The 
      linear range of detection in chicken extract was established at 0.025-3.500 ng/mL 
      with a half maximal inhibitory concentration at 0.22 +/- 0.02 ng/mL. Using the 
      optimized parameters, the limit of detection (LOD) for ENR using the FNs ICTS 
      sensor was recorded at 0.02 ng/mL in chicken extract. This corresponds to 0.12 
      mug/kg chicken meat which is two (2) orders of magnitude better that the maximum 
      residue limits (MRLs) imposed in Japan (10 mug/kg) and three (3) orders of 
      magnitude better than those imposed in China. The intra- and inter-assay 
      coefficient of variations (CVs) were 6.04% and 12.96% at 0.5 ng/mL, 6.92% and 
      12.61% at 1.0 ng/mL, and 6.66% and 11.88% at 2.0 ng/mL in chicken extract, 
      respectively. The recoveries using the new FNs ICTS sensor from fifty (50) 
      ENR-spiked chicken samples showed a highly significant correlation (R(2) = 
      0.9693) with the commercial enzyme-linked immunosorbent assay (ELISA) kit. The 
      new FNs ICTS sensor is a simple, rapid, sensitive, accurate, and inexpensive 
      quantitative detection of ENR residues in chicken meat and extracts.
FAU - Huang, Xiaolin
AU  - Huang X
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang 330047, P R China.
FAU - Aguilar, Zoraida P
AU  - Aguilar ZP
FAU - Li, Huaiming
AU  - Li H
FAU - Lai, Weihua
AU  - Lai W
FAU - Wei, Hua
AU  - Wei H
FAU - Xu, Hengyi
AU  - Xu H
FAU - Xiong, Yonghua
AU  - Xiong Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130507
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Fluoroquinolones)
RN  - 0 (Organometallic Compounds)
RN  - 0 (Phenanthrolines)
RN  - 0 (Reagent Strips)
RN  - 22873-66-1 (tris-(1,10-phenanthroline)ruthenium)
RN  - 3DX3XEK1BN (Enrofloxacin)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Calibration
MH  - Chickens
MH  - Chromatography, Affinity/*instrumentation
MH  - Drug Residues/*analysis
MH  - Enrofloxacin
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluoroquinolones/*analysis
MH  - Food Contamination/analysis
MH  - Limit of Detection
MH  - Meat/*analysis
MH  - Nanoparticles/*chemistry
MH  - Organometallic Compounds/*chemistry
MH  - Phenanthrolines/*chemistry
MH  - Reagent Strips/chemistry
MH  - Reproducibility of Results
MH  - Silicon Dioxide/*chemistry
EDAT- 2013/04/26 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/04/26 06:00
PHST- 2013/04/26 06:00 [entrez]
PHST- 2013/04/26 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 10.1021/ac400502v [doi]
PST - ppublish
SO  - Anal Chem. 2013 May 21;85(10):5120-8. doi: 10.1021/ac400502v. Epub 2013 May 7.