PMID- 23619304
OWN - NLM
STAT- MEDLINE
DCOM- 20140206
LR  - 20240323
IS  - 1751-7370 (Electronic)
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 7
IP  - 9
DP  - 2013 Sep
TI  - Oxygen-dependent niche formation of a pyrite-dependent acidophilic consortium 
      built by archaea and bacteria.
PG  - 1725-37
LID - 10.1038/ismej.2013.64 [doi]
AB  - Biofilms can provide a number of different ecological niches for microorganisms. 
      Here, a multispecies biofilm was studied in which pyrite-oxidizing microbes are 
      the primary producers. Its stability allowed not only detailed fluorescence in 
      situ hybridization (FISH)-based characterization of the microbial population in 
      different areas of the biofilm but also to integrate these results with oxygen 
      and pH microsensor measurements conducted before. The O2 concentration declined 
      rapidly from the outside to the inside of the biofilm. Hence, part of the 
      population lives under microoxic or anoxic conditions. Leptospirillum 
      ferrooxidans strains dominate the microbial population but are only located in 
      the oxic periphery of the snottite structure. Interestingly, archaea were 
      identified only in the anoxic parts of the biofilm. The archaeal community 
      consists mainly of so far uncultured Thermoplasmatales as well as novel ARMAN 
      (Archaeal Richmond Mine Acidophilic Nanoorganism) species. Inductively coupled 
      plasma analysis and X-ray absorption near edge structure spectra provide further 
      insight in the biofilm characteristics but revealed no other major factors than 
      oxygen affecting the distribution of bacteria and archaea. In addition to 
      catalyzed reporter deposition FISH and oxygen microsensor measurements, 
      microautoradiographic FISH was used to identify areas in which active CO2 
      fixation takes place. Leptospirilla as well as acidithiobacilli were identified 
      as primary producers. Fixation of gaseous CO2 seems to proceed only in the outer 
      rim of the snottite. Archaea inhabiting the snottite core do not seem to 
      contribute to the primary production. This work gives insight in the ecological 
      niches of acidophilic microorganisms and their role in a consortium. The data 
      provided the basis for the enrichment of uncultured archaea.
FAU - Ziegler, Sibylle
AU  - Ziegler S
AD  - Department of Microbiology, Albert-Ludwigs University, Freiburg, Germany.
FAU - Dolch, Kerstin
AU  - Dolch K
FAU - Geiger, Katharina
AU  - Geiger K
FAU - Krause, Susanne
AU  - Krause S
FAU - Asskamp, Maximilian
AU  - Asskamp M
FAU - Eusterhues, Karin
AU  - Eusterhues K
FAU - Kriews, Michael
AU  - Kriews M
FAU - Wilhelms-Dick, Dorothee
AU  - Wilhelms-Dick D
FAU - Goettlicher, Joerg
AU  - Goettlicher J
FAU - Majzlan, Juraj
AU  - Majzlan J
FAU - Gescher, Johannes
AU  - Gescher J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130425
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sulfides)
RN  - 132N09W4PR (pyrite)
RN  - E1UOL152H7 (Iron)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Aerobiosis
MH  - Anaerobiosis
MH  - Archaea/genetics/*physiology
MH  - Bacteria/genetics
MH  - *Bacterial Physiological Phenomena
MH  - *Biodiversity
MH  - *Biofilms
MH  - Carbon Cycle
MH  - Hydrogen-Ion Concentration
MH  - Iron/*metabolism
MH  - Microbial Consortia/*physiology
MH  - Mining
MH  - Oxygen/*metabolism
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sulfides/*metabolism
PMC - PMC3749503
EDAT- 2013/04/27 06:00
MHDA- 2014/02/07 06:00
PMCR- 2014/09/01
CRDT- 2013/04/27 06:00
PHST- 2012/10/31 00:00 [received]
PHST- 2013/03/01 00:00 [revised]
PHST- 2013/03/11 00:00 [accepted]
PHST- 2013/04/27 06:00 [entrez]
PHST- 2013/04/27 06:00 [pubmed]
PHST- 2014/02/07 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - ismej201364 [pii]
AID - 10.1038/ismej.2013.64 [doi]
PST - ppublish
SO  - ISME J. 2013 Sep;7(9):1725-37. doi: 10.1038/ismej.2013.64. Epub 2013 Apr 25.