PMID- 23619565
OWN - NLM
STAT- MEDLINE
DCOM- 20140305
LR  - 20231213
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 58
DP  - 2013 Aug
TI  - Barrier protective effects of piperlonguminine in LPS-induced inflammation in 
      vitro and in vivo.
PG  - 149-57
LID - S0278-6915(13)00270-6 [pii]
LID - 10.1016/j.fct.2013.04.027 [doi]
AB  - Piperlonguminine (PL), an important component of Piper longum fruits, is well 
      known to possess potent anti-hyperlipidemic, anti-platelet and anti-melanogenesis 
      activities. In this study, we first investigated the possible barrier protective 
      effects of piperlonguminine against proinflammatory responses induced by 
      lipopolysaccharide (LPS) and the associated signaling pathways in vitro and in 
      vivo. The barrier protective activities of PL were determined by measuring 
      permeability, monocytes adhesion and migration, and activation of proinflammatory 
      proteins in LPS-activated human umbilical vein endothelial cells (HUVECs) and in 
      mice. We found that PL inhibited LPS-induced barrier disruption, expression of 
      cell adhesion molecules (CAMs) and adhesion/transendothelial migration of 
      monocytes to human endothelial cells. PL also suppressed LPS-induced 
      hyperpermeability and leukocytes migration in vivo. Further studies revealed that 
      PL suppressed the production of tumor necrosis factor-alpha (TNF-alpha) or Interleukin 
      (IL)-6 and activation of nuclear factor-kappaB (NF-kappaB) or extracellular regulated 
      kinases (ERK) 1/2 by LPS. Moreover, treatment with PL resulted in reduced 
      LPS-induced septic mortality. Collectively, these results suggest that PL 
      protects vascular barrier integrity by inhibiting hyperpermeability, expression 
      of CAMs, adhesion and migration of leukocytes, thereby endorsing its usefulness 
      as a therapy for vascular inflammatory diseases.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Lee, Wonhwa
AU  - Lee W
AD  - College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook 
      National University, Daegu 702-701, Republic of Korea.
FAU - Yoo, Hayoung
AU  - Yoo H
FAU - Kim, Jeong Ah
AU  - Kim JA
FAU - Lee, Sangkyu
AU  - Lee S
FAU - Jee, Jun-Goo
AU  - Jee JG
FAU - Lee, Min Young
AU  - Lee MY
FAU - Lee, You-Mie
AU  - Lee YM
FAU - Bae, Jong-Sup
AU  - Bae JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130422
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Dioxolanes)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - SGD66V4SVJ (piperlongumine)
SB  - IM
MH  - Animals
MH  - Capillary Permeability/drug effects
MH  - Cells, Cultured
MH  - Dioxolanes/*pharmacology
MH  - Endothelium, Vascular/drug effects
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Inflammation/chemically induced/*prevention & control
MH  - Interleukin-6/biosynthesis
MH  - Lipopolysaccharides/*toxicity
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis
OTO - NOTNLM
OT  - Barrier integrity
OT  - Endothelium
OT  - Inflammation
OT  - Lipopolysaccharide
OT  - Piperlonguminine
EDAT- 2013/04/27 06:00
MHDA- 2014/03/07 06:00
CRDT- 2013/04/27 06:00
PHST- 2013/01/13 00:00 [received]
PHST- 2013/03/27 00:00 [revised]
PHST- 2013/04/12 00:00 [accepted]
PHST- 2013/04/27 06:00 [entrez]
PHST- 2013/04/27 06:00 [pubmed]
PHST- 2014/03/07 06:00 [medline]
AID - S0278-6915(13)00270-6 [pii]
AID - 10.1016/j.fct.2013.04.027 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2013 Aug;58:149-57. doi: 10.1016/j.fct.2013.04.027. Epub 2013 
      Apr 22.