PMID- 23620078
OWN - NLM
STAT- MEDLINE
DCOM- 20140908
LR  - 20140127
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 34
IP  - 3
DP  - 2014 Mar
TI  - Sex differences in blood genotoxic and cytotoxic effects as a consequence of 
      vanadium inhalation: micronucleus assay evaluation.
PG  - 258-64
LID - 10.1002/jat.2873 [doi]
AB  - Vanadium is an environmental pollutant attached to the smallest air suspended 
      particles that enters into the respiratory tract reaching the systemic 
      circulation. The oxidative state of this element and sex are factors related to 
      its toxicity. In this study, we explored sex-associated genotoxic and cytotoxic 
      differences in a mouse experimental model. Mice inhaled V2O5 (0.02 M) 2 h/twice a 
      week; blood samples were obtained at 24 h and every week until the end of the 
      4-week exposure. Samples were processed for fluorochrome-mediated viability and a 
      micronucleus assay in slides pre-covered with acridine orange (AO). The results 
      showed that males were more susceptible to genotoxicity during the exposure in 
      contrast to the females. In peripheral blood leukocytes, no cytotoxic differences 
      were observed in both, females or males, but the decrease in circulating 
      reticulocytes provides evidence of the metal's cytotoxic effect on the bone 
      marrow (BM). A significant decrease in reticulocytes was observed during the 
      experiment independent of the animal's sex. The present findings might be 
      explained by the interaction of the metal with the enzymes that control 
      erythropoiesis or a direct effect on erythropoietin production might explain our 
      findings; however, an absence of the genotoxic effects in females could be a 
      consequence of the protective effect against oxidative stress by their higher 
      estrogen levels. This study contributes to a better understanding of the 
      mechanisms by which vanadium induces adverse effects in biological systems.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - Rojas-Lemus, Marcela
AU  - Rojas-Lemus M
AD  - Departamento de Biologia Celular y Tisular, Facultad de Medicina, Universidad 
      Nacional Autonoma de Mexico (UNAM), Mexico, DF, 04510.
FAU - Altamirano-Lozano, Mario
AU  - Altamirano-Lozano M
FAU - Fortoul, Teresa I
AU  - Fortoul TI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130426
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Environmental Pollutants)
RN  - 0 (Vanadium Compounds)
RN  - BVG363OH7A (vanadium pentoxide)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Environmental Pollutants/*toxicity
MH  - Female
MH  - Inhalation Exposure/*adverse effects
MH  - Leukocytes/cytology/*drug effects
MH  - Male
MH  - Mice
MH  - Micronuclei, Chromosome-Defective/*chemically induced/statistics & numerical data
MH  - Micronucleus Tests
MH  - Reticulocytes/cytology/*drug effects
MH  - *Sex Characteristics
MH  - Vanadium Compounds/*toxicity
OTO - NOTNLM
OT  - cytotoxicity
OT  - genotoxicity
OT  - micronucleus
OT  - reticulocytes
OT  - sex differences
OT  - vanadium pentoxide
EDAT- 2013/04/27 06:00
MHDA- 2014/09/10 06:00
CRDT- 2013/04/27 06:00
PHST- 2012/11/12 00:00 [received]
PHST- 2013/02/11 00:00 [revised]
PHST- 2013/02/11 00:00 [accepted]
PHST- 2013/04/27 06:00 [entrez]
PHST- 2013/04/27 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
AID - 10.1002/jat.2873 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2014 Mar;34(3):258-64. doi: 10.1002/jat.2873. Epub 2013 Apr 26.