PMID- 23620790
OWN - NLM
STAT- MEDLINE
DCOM- 20131111
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 4
DP  - 2013
TI  - CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell 
      adhesion and migration.
PG  - e61761
LID - 10.1371/journal.pone.0061761 [doi]
LID - e61761
AB  - Activated chemokine receptor initiates inside-out signaling to transiently 
      trigger activation of integrins, a process involving multiple components that 
      have not been fully characterized. Here we report that GM-CSF/IL-3/IL-5 receptor 
      common beta-chain-associated protein (CBAP) is required to optimize this 
      inside-out signaling and activation of integrins. First, knockdown of CBAP 
      expression in human Jurkat T cells caused attenuated CXC chemokine ligand-12 
      (CXCL12)-induced cell migration and integrin alpha4beta1- and alphaLbeta2-mediated cell 
      adhesion in vitro, which could be rescued sufficiently upon expression of murine 
      CBAP proteins. Freshly isolated CBAP-deficient primary T cells also exhibited 
      diminution of chemotaxis toward CC chemokine ligand-21 (CCL21) and CXCL12, and 
      these chemokines-induced T-cell adhesions in vitro. Adoptive transfer of isolated 
      naive T cells demonstrated that CBAP deficiency significantly reduced lymph node 
      homing ability in vivo. Finally, migration of T cell-receptor-activated T cells 
      induced by inflammatory chemokines was also attenuated in CBAP-deficient cells. 
      Further analyses revealed that CBAP constitutively associated with both integrin 
      beta1 and ZAP70 and that CBAP is required for chemokine-induced initial binding of 
      the talin-Vav1 complex to integrin beta1 and to facilitate subsequent ZAP70-mediated 
      dissociation of the talin-Vav1 complex and Vav1 phosphorylation. Within such an 
      integrin signaling complex, CBAP likely functions as an adaptor and ultimately 
      leads to activation of both integrin alpha4beta1 and Rac1. Taken together, our data 
      suggest that CBAP indeed can function as a novel signaling component within the 
      ZAP70/Vav1/talin complex and plays an important role in regulating 
      chemokine-promoted T-cell trafficking.
FAU - Chiang, Yun-Jung
AU  - Chiang YJ
AD  - Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, 
      Taiwan.
FAU - Ho, Kun-Chin
AU  - Ho KC
FAU - Sun, Chien-Tsang
AU  - Sun CT
FAU - Chiu, Jeng-Jiann
AU  - Chiu JJ
FAU - Lee, Fang-Jen
AU  - Lee FJ
FAU - Liao, Fang
AU  - Liao F
FAU - Yang-Yen, Hsin-Fang
AU  - Yang-Yen HF
FAU - Yen, Jeffrey Jong-Young
AU  - Yen JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130419
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Chemokines)
RN  - 0 (Integrin beta1)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proto-Oncogene Proteins c-vav)
RN  - 0 (TMEM102 protein, human)
RN  - 0 (TMEM102 protein, mouse)
RN  - 0 (Talin)
RN  - 0 (VAV1 protein, human)
RN  - EC 2.7.10.2 (ZAP-70 Protein-Tyrosine Kinase)
RN  - EC 2.7.10.2 (ZAP70 protein, human)
RN  - EC 3.6.5.2 (rac1 GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Movement/*drug effects
MH  - Chemokines/*pharmacology
MH  - Chemotaxis/drug effects
MH  - Humans
MH  - Integrin beta1/metabolism
MH  - Jurkat Cells
MH  - Lymph Nodes/cytology/drug effects
MH  - Lymphocyte Activation/drug effects
MH  - Membrane Proteins/deficiency/*metabolism
MH  - Mice
MH  - Protein Binding/drug effects
MH  - Proto-Oncogene Proteins c-vav
MH  - Signal Transduction/drug effects
MH  - T-Lymphocytes/*cytology/drug effects/*metabolism
MH  - Talin/metabolism
MH  - ZAP-70 Protein-Tyrosine Kinase/*metabolism
MH  - rac1 GTP-Binding Protein/metabolism
PMC - PMC3631140
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/04/27 06:00
MHDA- 2013/11/12 06:00
PMCR- 2013/04/19
CRDT- 2013/04/27 06:00
PHST- 2013/01/02 00:00 [received]
PHST- 2013/03/11 00:00 [accepted]
PHST- 2013/04/27 06:00 [entrez]
PHST- 2013/04/27 06:00 [pubmed]
PHST- 2013/11/12 06:00 [medline]
PHST- 2013/04/19 00:00 [pmc-release]
AID - PONE-D-13-00685 [pii]
AID - 10.1371/journal.pone.0061761 [doi]
PST - epublish
SO  - PLoS One. 2013 Apr 19;8(4):e61761. doi: 10.1371/journal.pone.0061761. Print 2013.